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Data and Publications
(A) The biological activity of Human Recombinant MCP-2 (CCL8) was tested using CHO cells transfected with human CCR5. Calcium mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of CCL8 where calcium mobilization is at 50% of maximum. The EC50 in the example above is less than 0.5 μg/mL. (B) 2 μg of Human Recombinant MCP-2 (CCL8) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant MCP-2 (CCL8) has a predicted molecular mass of 8.9 kDa.