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Data and Publications
(A) The biological activity of Human Recombinant LIF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell
proliferation is at 50% of maximum. The EC50 for this effect is 0.09 ng/mL.
(B) 2 μg of Human Recombinant LIF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant LIF has a predicted molecular mass of 19.7 kDa.