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Data and Publications
(A) The biological activity of Human Recombinant IL-8 (CXCL8) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCXCR1 cells (human Gα15 and human CXCR1 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.15 μg/mL. (B) 2 μg of Human Recombinant IL-8 (CXCL8) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-8 (CXCL8) has a predicted molecular mass of 8.9 kDa.