Human Recombinant IL-8 (CXCL8)

Interleukin 8

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Interleukin 8
From: 82 USD

Overview

Interleukin-8 (IL-8) is a member of the CXC subfamily of chemokines and is produced by leukocytic cells (monocytes, T cells, neutrophils, and natural killer cells) and non-leukocytic somatic cells (endothelial cells, fibroblasts, and epithelial cells), with the most prominent source being monocytes and macrophages. Its production is induced by inflammatory stimuli, such as IL-1. IL-8, also known as CXCL8, activates neutrophils inducing chemotaxis, exocytosis, and the respiratory burst (Baggiolini & Clark-Lewis; Mukaida). IL-8 is considered one of the most potent neutrophil chemoattractants in inflammation and binds to two different chemokine receptors on leukocytes: the G protein-coupled receptors CXCR1 and CXCR2 (Hoffmann et al.; de Oliveira et al.). IL-8 has angiogenic effects on human intestinal microvascular endothelial cells in vitro that are mediated by CXCR2 (Heidemann et al.). IL-8 is reported to promote breast cancer progression by increasing cell invasion, angiogenesis, and metastasis and has been reported to be involved in regulating breast cancer stem-like cells (Singh et al.). IL-8 also has proangiogenic properties in inflammatory diseases of the conjunctiva, cornea, iris, retina, and orbit (Ghasemi et al.). It was also shown that a major T cell effector function in human newborns is IL-8 production, which has the potential to activate antimicrobial neutrophils and gamma/delta T cells (Gibbons et al.). A variety of human pathogens, such as HIV and Mycobacterium tuberculosis, have been shown to induce IL-8 production by monocytes and macrophages (Friedland et al.; Meddows-Taylor et al.).
Alternative Names:
CXC motif ligand 8, GCP-1, Granulocyte chemotactic protein 1, Interleukin-8, MDNCF, Monocyte-derived neutrophil chemotactic factor, NAF, NAP-1, Neutrophil activating factor, SCYB8, Small inducible cytokine subfamily B member 8
Cell Type:
Airway Cells; Endothelial Cells; Keratinocytes; Macrophages; Monocytes; NK Cells
Species:
Human
Area of Interest:
Epithelial Cell Biology; Immunology; Stem Cell Biology
Molecular Weight:
8.9 kDa
Purity:
≥ 95 %

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Data and Publications

Data

(A) The biological activity of Human Recombinant IL-8 (CXCL8) was tested by its ability to mobilize Ca2+ in CHO-K1/Gα15/hCXCR1 cells (human Gα15 and human CXCR1 stably expressed in CHO-K1 cells). Ca2+ mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.15 μg/mL. (B) 2 μg of Human Recombinant IL-8 (CXCL8) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-8 (CXCL8) has a predicted molecular mass of 8.9 kDa.

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