Human Recombinant GM-CSF (CHO-expressed)

Granulocyte-macrophage colony-stimulating factor

Human Recombinant GM-CSF (CHO-expressed)

Granulocyte-macrophage colony-stimulating factor

From: 243 USD
Catalog #
78190_C
Granulocyte-macrophage colony-stimulating factor

Overview

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils, and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and used to treat chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).
Subtype
Cytokines
Alternative Names
CF, Colony-stimulating factor, CSF, CSF2, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Monocytes, Myeloid Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
14.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78190, 78190.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78190, 78190.1
Lot #
All
Language
English

Data and Publications

(A) The biological activity of Human Recombinant GM-CSF (CHO-expressed) was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.2 ng/mL. (B) 2 μg of Human Recombinant GM-CSF (CHO-expressed) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GM-CSF (CHO-expressed) has a predicted molecular mass of 14.5 kDa.

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