Human Recombinant Flt3/Flk-2 Ligand

Fms-like tyrosine kinase 3/fetal liver kinase-2
Catalog #
78009_C
Fms-like tyrosine kinase 3/fetal liver kinase-2
From: 185 USD

Overview

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) Ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and in the development of the immune system (Hannum et al.). Flt3/Flk-2 Ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive CD34+ hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 Ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with the interleukins IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, and GM-CSF and TNF-α, Flt3/Flk-2 Ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 Ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler & Quentmeier; Wodnar-Filipowicz).

Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.).
Subtype
Cytokines
Alternative Names
FL, FLT3L, Flt3-L, Flt3 Ligand, Fms-like tyrosine kinase 3 ligand
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, NK Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
17.6 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
Human Recombinant Flt3/Flk-2 Ligand
Catalog #
78009, 78009.1, 78009.3
Lot #
BX22547 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
Human Recombinant Flt3/Flk-2 Ligand
Catalog #
78009, 78009.1, 78009.3
Lot #
BX22547 and higher
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant Flt3/Flk-2 Ligand
Catalog #
78009, 78009.1, 78009.3
Lot #
All
Language
English

Educational Materials (3)

Brochure
Products for Human Pluripotent Stem Cells
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand was tested by its ability to promote the proliferation of OCI-AML5 cells. Cell proliferation was measured after 60 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.7 - 2.5 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 17.6 kDa.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.