Human Recombinant Flt3/Flk-2 Ligand
Overview
Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.).
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet 1
|
Product Name
Human Recombinant Flt3/Flk-2 Ligand
|
Catalog #
78009, 78009.1, 78009.3 |
Lot #
BX22547 and higher |
Language
English |
Document Type
Product Information Sheet 2
|
Product Name
Human Recombinant Flt3/Flk-2 Ligand
|
Catalog #
78009, 78009.1, 78009.3 |
Lot #
BX22547 and higher |
Language
English |
Document Type
Safety Data Sheet
|
Product Name
Human Recombinant Flt3/Flk-2 Ligand
|
Catalog #
78009, 78009.1, 78009.3 |
Lot #
All |
Language
English |
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand was tested by its ability to promote the proliferation of OCI-AML5
cells. Cell proliferation was measured after 60 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.7 - 2.5 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 17.6 kDa.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.