Human Recombinant Flt3/Flk-2 Ligand, ACF

Fms-like tyrosine kinase 3/fetal liver kinase-2, animal component-free
Catalog #
78137_C
Fms-like tyrosine kinase 3/fetal liver kinase-2, animal component-free
From: 204 USD

Overview

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) Ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and the development of the immune system (Hannum et al.). Flt3/Flk-2 Ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive CD34+ hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 Ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with the interleukins IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, and GM-CSF and TNF-α, Flt3/Flk-2 Ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 Ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler & Quentmeier; Wodnar-Filipowicz).


Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
FL, FLT3L, Flt3-L, Flt3 Ligand, Fms-like tyrosine kinase 3 ligand
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, NK Cells
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
17.6 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant Flt3/Flk-2 Ligand, ACF
Catalog #
78137, 78137.1, 78137.2
Lot #
BX22356 and higher
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant Flt3/Flk-2 Ligand, ACF
Catalog #
78137, 78137.1, 78137.2
Lot #
All
Language
English

Educational Materials (6)

Brochure
Small Molecules for Stem Cell Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Webinar
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
1:06:52
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand, ACF was tested by its ability to promote the proliferation of OCI-AML5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.498 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand, ACF has a predicted molecular mass of 17.6 kDa.

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