Mouse Recombinant SCF (P. pastoris-expressed)

Stem cell factor

Mouse Recombinant SCF (P. pastoris-expressed)

Stem cell factor

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Stem cell factor
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Overview

Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Huang et al. Cell, 1990; Kent et al. Clin Cancer Res, 2008). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy. Cell, 1997). In the mouse, SCF is essential during fetal gonadal development (Mauduit. Hum Reprod Update, 1999). It is produced by stromal cells in the fetal liver, bone marrow, and thymus, in keratinocytes, and in the central nervous system and gut mucosa, and can function as a chemotactic and chemokinetic factor.

SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit has also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit-positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain (Lennartsson & Ronnstrand. Physiol Rev, 2012).
Subtype
Cytokines
Alternative Names
Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Mouse
Area of Interest
Stem Cell Biology
Purity
≥ 95%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Mouse Recombinant SCF (P. pastoris-expressed)

(A) The biological activity of Mouse Recombinant SCF (P. pastoris-expressed) was tested by its ability to promote the proliferation of human TF-1 cells. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is ≤ 10 ng/mL. (B) 2 μg Mouse Recombinant SCF (P. pastoris-expressed) was resolved with SDS-PAGE under non-reducing (-) and reducing (+) conditions. Mouse Recombinant SCF has a predicted molecular mass of 18.4 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1715, 100-1714
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1715
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1714
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.