Figure 1. Biological Activity and Molecular Mass of Human Recombinant Noggin (E. coli-expressed), ACF

(A) The biological activity of Human Recombinant Noggin (E. coli-expressed), ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells. Inhibition of BMP-2 was measured using a firefly luciferase reporter assay. Cells were treated with a serial dilution of Noggin and standard concentration of BMP-2 for 6 hours. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.5 nM (70.1 ng/mL). (B) 7 μg of Human Recombinant Noggin (E. coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Noggin has a predicted molecular mass of 23 kDa in reducing conditions.