Human Recombinant HGF (E. coli-expressed), ACF

Hepatocyte growth factor, animal component-free

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Human Recombinant HGF (E. coli-expressed), ACF

Hepatocyte growth factor, animal component-free

From: 699 USD

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Hepatocyte growth factor, animal component-free

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Overview

Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic growth factor that promotes proliferation, motility, survival, and differentiation. HGF binds and promotes the dimerization and activation of the receptor tyrosine kinase c-MET, and stimulates PI3K/AKT, FAK, JNK, and ERK1/2 signaling (Organ & Tsao. Ther Adv Med Oncol, 2011). HGF stimulates migration of cells during embryogenesis, induces cell motility and scattering of epithelial cells, and regulates epithelial-mesenchymal transition. It is a chemoattractant for motor neurons, and regulates the development of sensory, sympathetic, parasympathetic, and cortical neurons as well as the proliferation of oligodendrocytes and glial development. Additionally, HGF is cytoprotective and regulates liver growth and regeneration (Nakamura et al. J Gastroenterol Hepatol, 2011).

Cytokines

DFNB39, F-TCF, Fibroblast-derived tumor cytotoxic factor, Hepapoietin A, HPTA, Lung fibroblast-derived mitogen, Scatter factor, SF

Airway Cells, Hepatic Cells, Intestinal Cells, Mammary Cells, Neurons, Pluripotent Stem Cells, Prostate Cells

Human

Epithelial Cell Biology, Neuroscience, Stem Cell Biology

Animal Component-Free

≥ 98%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Human Recombinant HGF (E. coli-expressed), ACF

(A) The biological activity of Human Recombinant HGF (E.coli-expressed), ACF was tested by its ability to induce MAP/ERK signaling in transfected HEK293T cells. Cells were treated with a serial dilution of HGF for 6 hours, and the activity was quantified by a luciferase reporter assay. The EC50 is defined as the effective concentration of the growth factor at which MAP/ERK activity is at 50% of maximum. The EC50 in the above example is ≤ 49.6 pM (0.99 ng/mL). (B) 7 μg of Human Recombinant HGF (E.coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant HGF has a predicted molecular mass of 21 kDa in reducing conditions and 18 kDa in non-reducing conditions.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Human Recombinant HGF (E. coli-expressed), ACF

Catalog #

100-1727, 100-1728

Lot #

All

Language

English

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Safety Data Sheet

Product Name

Human Recombinant HGF (E. coli-expressed), ACF

Catalog #

100-1727

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

Human Recombinant HGF (E. coli-expressed), ACF

Catalog #

100-1728

Lot #

All

Language

English

Resources and Publications

Related Products

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

Human Recombinant HGF (E. coli-expressed), ACF

Hepatocyte growth factor, animal component-free

Human Recombinant HGF (E. coli-expressed), ACF

Hepatocyte growth factor, animal component-free

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Overview

Data Figures

Protocols and Documentation

Resources and Publications

Educational Materials (4)

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