48 Inserts; 4 x 24-well plates, each with 12 inserts
Data and Publications
Figure 1. Representative Images of ALI Culture Morphology
A side-by-side comparison was performed between Transwell® Inserts and competitor inserts of the same material and pore size. HBECs were seeded onto the inserts at passage 3 (P3) and differentiated in PneumaCult™-ALI for 21 days. Air-liquid interface (ALI) cultures generated using PneumaCult™ and Transwell® Inserts provided the most optimal morphology.
Figure 2. Differential Epithelial Cell Marker Expression in ALI Cultures Generated with Different Inserts
HBECs were seeded onto the inserts at P3 and differentiated in PneumaCult™-ALI for 21 days. Gene expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers was assessed by qPCR and normalized to beta actin. Comparison was made with competitor inserts of the same material and pore size. Higher expression of goblet and ciliated cell markers was observed when using PneumaCult™ together with Transwell® inserts.