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MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

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MesenCult™-ACF Freezing Medium

Animal component-free MSC cryopreservation medium

From: 262 USD
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Animal component-free MSC cryopreservation medium
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Product Advantages


  • Serum-free and animal component-free

  • Reproducibly high recovery rates

  • Optimized for MSCs previously cultured in MesenCult™-ACF Plus Medium or MesenCult™ Medium

  • Preserves human MSC multipotency and expansion capacities

  • Convenient, ready-to-use format

Overview

MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.
Contains
Dimethyl sulfoxide (DMSO)
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
Cryopreservation
Brand
MesenCult
Area of Interest
Stem Cell Biology
Formulation Category
Animal Component-Free, Serum-Free

Data Figures

Human MSCs previously cultured in animal component-free medium and cryopreserved in MesenCult™-ACF Freezing Medium show reproducibly high recovery of viable cells after thawing. Recovery is higher than observed with a competitor ACF freezing medium (n = 6 independent experiments, P = 0.0035). Viable cell recovery is defined as the percentage of input cells that are recovered and maintain post-thaw viability.

Human MSCs cryopreserved with MesenCult™-ACF Freezing Medium (A, 3 days after thawing) maintain multi-lineage differentiation potential, as demonstrated by differentiation to the adipogenic (B, Oil Red O staining) and osteogenic (C, Alizarin Red staining) lineages.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Freezing Medium
Catalog #
05490
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
MesenCult™-ACF Freezing Medium
Catalog #
05490
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (7)

Products for Your Mesenchymal Stromal Cell Research
Brochure
Products for Your Mesenchymal Stromal Cell Research
Cryopreservation Media for Stem Cell Research
Brochure
Cryopreservation Media for Stem Cell Research
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
13:45
Video
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
1:51
Video
MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Tools to Accelerate Your MSC and EV Workflows
30:05
Webinar
Tools to Accelerate Your MSC and EV Workflows
The Secret In Vivo Life of "Mesenchymal Stem Cells"
58:00
Webinar
The Secret In Vivo Life of "Mesenchymal Stem Cells"

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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