Cell Storage Media

Freeze Your Cells with Our Optimized Cryopreservation Media

Storage and cryopreservation of cells and tissues are vital for your biological research workflow. Along with defined and serum-free cryopreservation media optimized for cells cultured in STEMCELL’s media, we offer a suite of cGMP-manufactured, protein-free and serum-free cryopreservation products that are designed to maintain high cell viability and maximize cell recovery after long-term storage.

cGMP-Manufactured Preservation Media Formulated with USP-Grade Components:

CryoStor® Freezing Media

Cell Types:

  • Cord blood and tissue, peripheral blood, mesenchymal stem cells, pluripotent stem cells, tissue samples and others

Recommended For:

  • Cryopreservation of hepatocytes, tissue samples, human peripheral blood, human mesenchymal stem cells, human embryonic and human induced pluripotent stem cells (human ES cells and iPS cells) and other extremely sensitive cell types

Features:

  • Designed to mitigate temperature-induced molecular stress responses during freezing and thawing

Formulation:

  • Pre-formulated with 2%, 5%, or 10% USP-grade DMSO
  • Manufactured under cGMP

BloodStor® Freezing Media

Cell Types:

  • Cord blood and tissue, peripheral blood and bone marrow

Recommended For:

  • Cryopreservation of stem cells and other cells isolated from umbilical cord blood, peripheral blood, bone marrow, and other biologics

Features:

  • Formulated to be compatible with many automated stem cell banking systems

Formulation:

  • BloodStor® 55-5 is preformulated with 55% (w/v) DMSO USP, 5% (w/v) Dextran-40 USP and water for injection (WFI) quality water
  • BloodStor® 100 contains 100% (w/v) DMSO USP
  • Manufactured under cGMP

HypoThermosol® FRS Preservation Media

Cell Types:

  • All cells and tissues including pluripotent stem cells, hematopoietic stem and progenitor cells, and mesenchymal stem cells

Recommended For:

  • Short-term storage and/or shipment of cells at 2 – 8°C rather than at cryogenic temperatures

Features:

  • Uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process

Formulation:

  • HypoThermosol® FRS Preservation Media is a serum-free, protein-free and animal component-free medium
  • Manufactured under cGMP
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Defined and Serum-Free Cryopreservation Media:

mFreSR™ and FreSR-S™ Cryopreservation Media

Cell Types:

  • Human embryonic stem (ES) and induced pluripotent stem (iPS) cells

Recommended For:

  • Cryopreservation of ES and iPS cells cultured in TeSR™ media

Features:

  • Higher thawing efficiencies than with conventional methods using serum1-4
  • FreSR™-S is optimized for cryopreservation of cells in single cell suspension

Formulation:

  • FreSR™-S is an animal component-free (ACF) medium, optimized for cryopreservation of cells in single cell suspension

MesenCult™-ACF Cryopreservation Medium

Cell Types:

  • Mesenchymal stem cells (MSCs)

Recommended For:

  • Cryopreservation of MSCs, including human MSCs previously cultured in MesenCult™-XF or MesenCult™-ACF media

Features:

  • Thawed MSCs have reproducibly high recovery rates and maintain MSC multipotency and expansion capacities

Formulation:

  • MesenCult™-ACF Freezing Medium is a defined, serum-free and animal component-free medium that contains DMSO

STEMdiff™ Neural Progenitor Freezing Medium

Cell Types:

  • Neural progenitor cells (NPCs) derived from human pluripotent stem cells

Recommended For:

  • Cryopreservation of NPCs generated from ES or iPS cells using STEMdiff™ Neural Induction Medium

Features:

  • NPCs can be frozen at any point post-neural induction with reproducibly high recovery rates
  • Post-thaw, NPCs display healthy morphology, express NPC markers and retain the potential to expand and differentiate into neurons, astrocytes and other neural cell types

Formulation:

  • STEMdiff™ Neural Progenitor Freezing Medium as a defined and serum-free medium containing DMSO
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Data

Immune Cells Cryopreserved in CryoStor®CS10 Show Reproducibly High Post-Thaw Cell Viability

Figure 1. Immune Cells Cryopreserved in CryoStor®CS10 Show Reproducibly High Post-Thaw Cell Viability

CryoStor®CS10 effectively mitigates temperature-induced molecular cell stress responses to maximize post-thaw viability and recovery for a variety of immune cell types, including T cells (data not shown) and B cells. Here, human B cells from 6 different donors cryopreserved in CryoStor®CS10 show reproducibly high viability after thawing, as measured by Propidium Iodide staining (ranging from 94.3 - 97.9%).

Immune Cells Cryopreserved in CryoStor®CS10 Retain Functionality Post-Thaw

Figure 2. Immune Cells Cryopreserved in CryoStor®CS10 Retain Functionality Post-Thaw

(A) Human peripheral blood Pan-T cells cryopreserved in CryoStor®CS10 were thawed and cultured with or without the addition of T cell activating factors. Cells from Donors 1-3 were cultured in RPMI Medium supplemented with 10% FBS, with (activated) or without (control) 40 ng/mL PMA and 1 ug/mL Ionomycin for 24 hours. Cells from Donors 4-5 were cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981), with (activated) or without (control) ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) for 48 hours. Supernatants were collected from the cultures, and concentrations of secreted cytokines were determined using the Human IL-2 ELISA Kit (Catalog #02006). Activation by either PMA and Ionomycin or ImmunoCult™ Human CD3/CD28 T Cell Activator led to increased secretion of IL-2 compared to unstimulated control cultures. (B) Human B cells (Donors 6 - 11) cryopreserved in CryoStor®CS10 were thawed and activated with 1 µg/mL CD40 and 100 ng/mL IL-21 for 7 days. Supernatants were collected from the cultures and immunoglobulin G (IgG) production was measured using the Human IgG ELISA Antibody Pair Kit (Catalog #01994). Compared to unstimulated control cultures, B cell activation led to increased IgG​ ​secretion.

mFreSR™ Improves Thawing Efficiencies 5- to 10-Fold over Other Reported Methods

Figure 3. mFreSR™ Improves Thawing Efficiencies 5- to 10-Fold over Other Reported Methods

H9 hESCs were cryopreserved in mFreSR™ at the indicated passage number. Thawing efficiencies were analyzed by counting the number of surviving clumps after thawing.

References

  1. Fujioka T, et al. (2004) A simple and efficient cryopreservation method for primate embryonic stem cells. Int J Dev Biol 48(10): 1149-54
  2. Ha SY, et al. (2005) Cryopreservation of human embryonic stem cells without the use of a programmable freezer. Hum Reprod 20(7): 1779-85
  3. Ji L, et al. (2004) Cryopreservation of adherent human embryonic stem cells. Biotechnol Bioeng 88(3): 299-312
  4. Ware CB, et al. (2005) Controlled-rate freezing of human ES cells. Biotechniques 38(6): 879-80, 882-3
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