EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection of particle-free mouse cells labeled with biotinylated antibodies

1 x 10^9 cells
Catalog # 17655
768 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Release Mouse Biotin Positive Selection Kit is designed to isolate cells that are labeled with biotin-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556), providing highly purified particle-free cells.
Advantages
• Highly purified cells labeled with biotinylated antibodies isolated from mouse tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
  • EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655)
    • EasySep™ Biotin Positive Cocktail, 0.5 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • Normal Rat Serum, 2 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Lymphocytes, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
1000006156 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
19D102085 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English

Educational Materials(7)

Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™ Results in Highly Purified, Functional and Particle-Free Cells

Data and Publications

Data

Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).

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