EasySep™ Release Mouse Biotin Positive Selection Kit
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EasySep™ MagnetMagnet for column-free immunomagnetic separation
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EasySep™ BufferCell separation buffer
Overview
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556), providing highly purified particle-free cells.
• No-wash removal of EasySep™ Releasable RapidSpheres™
- EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655)
- EasySep™ Biotin Positive Cocktail, 0.5 mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- Normal Rat Serum, 2 mL
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet 1
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
19D102085 or lower |
Language
English |
|
Document Type
Product Information Sheet 2
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
1000006156 and higher |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
|
Catalog #
17655 |
Lot #
All |
Language
English |
Educational Materials (7)
Data and Publications
Data
Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).
Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
