EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II
Overview
• Up to 93% purity
• No columns required
- EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Catalog #18783)
- EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
- EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
- EasySep™ PE Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 0.5 mL
- Rat Serum, 2 mL
- RoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Catalog #18783RF)
- EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
- EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
- EasySep™ PE Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 0.5 mL
- Rat Serum, 2 mLRoboSep™ Buffer (Catalog #20104)RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility• EasySep™ Magnet (Catalog #18000)• “The Big Easy” EasySep™ Magnet (Catalog #18001)• EasyEights™ Magnet (Catalog #18103)• RoboSep™-S (Catalog #21000)SubtypeCell Isolation KitsCell TypeT Cells, T Cells, RegulatorySpeciesMouseSample SourceSpleenBrandEasySep, RoboSepArea of InterestImmunology
Scientific Resources
Product Documentation
Educational Materials (9)
Frequently Asked Question
Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ Streptavidin RapidSphere™ separations be automated?
Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?
Can I alter the separation time in the magnet?
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data


Figure 1. FACS Histogram Results with EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II
Starting with mouse splenocytes, the regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction typically ranges from 70 - 93%. In the above example, the purities of the start and final isolated fractions are 2% and 72%, respectively.
Publications (4)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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