EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II
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EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II -
RoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II -
Label for EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II - BOX
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
• Up to 93% purity
• No columns required
- EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Catalog #18783)
- EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
- EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
- EasySep™ PE Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 0.5 mL
- Rat Serum, 2 mL
- RoboSep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (Catalog #18783RF)
- EasySep™ Mouse CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
- EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
- EasySep™ PE Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- EasySep™ Mouse FcR Blocker, 0.5 mL
- Rat Serum, 2 mLRoboSep™ Buffer (Catalog #20104)RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:• EasySep™ Magnet (Catalog #18000)• “The Big Easy” EasySep™ Magnet (Catalog #18001)• EasyEights™ Magnet (Catalog #18103)• RoboSep™-S (Catalog #21000)Subtype:Cell Isolation KitsCell Type:T Cells, Regulatory; T CellsSpecies:MouseSample Source:SpleenBrand:EasySep; RoboSepArea of Interest:Immunology
Related Products
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EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit
Immunomagnetic positive selection cell isolation kit -
EasySep™ Mouse T Cell Isolation Kit
15-Minute cell isolation kit using immunomagnetic negative selection
Labeling Antibodies
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Anti-Mouse CD4 Antibody, Clone RM4-4
Rat monoclonal IgG2b antibody against mouse CD4 -
Anti-Mouse CD25 Antibody, Clone 3C7
Rat monoclonal IgG2b antibody against mouse CD25 -
Anti-Mouse CD4 Antibody, Clone RM4-5
Rat monoclonal IgG2a antibody against mouse CD4
Scientific Resources
Product Documentation
Educational Materials
(9)
Frequently Asked Questions
Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ Streptavidin RapidSphere™ separations be automated?
Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?
Can I alter the separation time in the magnet?
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. FACS Histogram Results with EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II
Starting with mouse splenocytes, the regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction typically ranges from 70 - 93%. In the above example, the purities of the start and final isolated fractions are 2% and 72%, respectively.