EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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From: 644 USD

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Immunomagnetic positive selection cell isolation kit
From: 644 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit is designed to isolate CD138+ (syndecan-1) cells from whole blood and bone marrow by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).

This product replaces the EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit (Catalog #18387) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• No columns required
Components:
  • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887RF)
    • EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Myeloma; Plasma Cells
Species:
Human
Sample Source:
Bone Marrow; Whole Blood
Selection Method:
Positive
Application:
ELISA
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(10)
Brochure
HLA Antibodies for Purity Assessment
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138 Plasma Cells
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
1:41
RoboSep™-S Introduction
Video
1:23
How to Isolate Cells with EasySep™
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Webinar
53:41
Enhance Sensitivity of Downstream Assays with Highly Purified Cells for Multiple Myeloma and CLL Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from 83.7 - 98.3%. In the above example, the purities of the start and final isolated fractions are 9.1% and 90.4%, respectively. NOTE: For samples with CD138+ starting frequency < 10 - 15%, the CD138+ purity of the isolated fraction may be variable. NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.