EasySep™ Human Naïve Pan T Cell Isolation Kit

Immunomagnetic negative selection kit

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From: 756 USD

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Immunomagnetic negative selection kit
From: 756 USD

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Overview

The EasySep™ Human Naïve Pan T Cell Isolation Kit is designed to isolate naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components:
    • EasySep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Human Naïve Pan T Cell Isolation Kit (Catalog #17961RF)
        • EasySep™ Human Naïve Pan T Cell Isolation Cocktail, 1 mL
        • EasySep™ Human TCR Gamma/Delta Depletion Cocktail, 1 mL
        • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
        • RoboSep™ Buffer (Catalog #20104)
        • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(8)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with fresh PBMCs, the naïve pan T cell content (CD3+CD45RA+CD45RO-CD197+) of the isolated fraction is typically 96.1 ± 2.3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 4.5% and 97%, respectively.

Publications

(1)
Science (New York, N.Y.) 2019

LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling.

J. H. Choi et al.

Abstract

Precise control of Wnt signaling is necessary for immune system development. In this study, we detected severely impaired development of all lymphoid lineages in mice, resulting from an N-ethyl-N-nitrosourea-induced mutation in the limb region 1-like gene (Lmbr1l), which encodes a membrane-spanning protein with no previously described function in immunity. The interaction of LMBR1L with glycoprotein 78 (GP78) and ubiquitin-associated domain-containing protein 2 (UBAC2) attenuated Wnt signaling in lymphocytes by preventing the maturation of FZD6 and LRP6 through ubiquitination within the endoplasmic reticulum and by stabilizing destruction complex" proteins. LMBR1L-deficient T cells exhibited hallmarks of Wnt/beta-catenin activation and underwent apoptotic cell death in response to proliferative stimuli. LMBR1L has an essential function during lymphopoiesis and lymphoid activation acting as a negative regulator of the Wnt/beta-catenin pathway."
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.