EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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EasySep™ Human IgG+ Memory B Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

Catalog #17868
845 USD

Required Products

Overview

The EasySep™ Human IgG+ Memory B Cell Isolation Kit isolates IgG+CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells, as well as IgM+, IgD+ and IgA+ cells are depleted using EasySep™ Dextran RapidSpheres™. The final isolated fraction contains IgG+CD27+ memory B cells, which readily secrete IgG in culture supernatants in the presence of appropriate stimuli.
Advantages:
• Highly purified IgG+ memory B cells isolated in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Optional isolation of untouched naïve B cells from the same sample
Components:
  • EasySep™ Human IgG+ Memory B Cell Isolation Kit (Catalog #17868)
    • EasySep™ Human CD27 Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 2 x 1 mL
    • EasySep™ Release Buffer, 2 x 1 mL
    • EasySep™ Human Memory B Cell Pre-Enrichment Cocktail, 2 x 1 mL
    • EasySep™ Human IgM/IgD/IgA Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Workflow Stages

Data and Publications

Data

FACS Profile Results with EasySep™ Human IgG+ Memory B Cell Isolation Kit

Figure 1. EasySep™ Human IgG+ Memory B Cell Isolation Kit

Starting with PBMCs, the IgG+ memory B cell content (CD19+CD27+IgG+) of the isolated fraction is typically 82.6 ± 7.0% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and isolated fractions are 1.0% and 90.2% respectively.

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.