EasySep™ Human EpCAM Positive Selection Kit II

Immunomagnetic positive selection kit
Catalog #
17846_C
Immunomagnetic positive selection kit
From: 707 USD
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Required Products
  1. HBSS with 10 mM HEPES, Without Phenol Red
    HBSS with 10 mM HEPES, Without Phenol Red

    Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red

  2. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.

This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Advantages
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components
  • EasySep™ Human EpCAM Positive Selection Kit II (Catalog #17846)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human EpCAM Positive Selection Kit II with Filter Tips (Catalog #17846RF)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Mammary Cells
Species
Human
Sample Source
Other, Primary
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Cancer Research, Epithelial Cell Biology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human EpCAM Positive Selection Kit II
Catalog #
17846
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human EpCAM Positive Selection Kit II
Catalog #
17846RF
Lot #
All
Language
English

Educational Materials(5)

Brochure
Mammary Epithelial Cells: Standardized Media and Reagents
Brochure
EasySep™ Cell Separation Technology
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Scientific Poster
Column-Free Enrichment of EPCAM Positive and CD49F Positive Human Mammary Epithelial Progenitor Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.

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