EasySep™ Human Eosinophil Isolation Kit

11-Minute cell isolation kit using immunomagnetic negative selection

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Eosinophil Isolation Kit

11-Minute cell isolation kit using immunomagnetic negative selection

From: 904 USD
Catalog #
17956_C
11-Minute cell isolation kit using immunomagnetic negative selection

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 99% purity

  • Untouched, viable cells

What's Included

  • EasySep™ Human Eosinophil Isolation Kit (Catalog #17956)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human Eosinophil Isolation Kit with Filter Tips (Catalog #17956RF)
    • EasySep™ Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Human Eosinophil Isolation Kit is designed to isolate eosinophils from a polymorphonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-eosinophils and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The kit is compatible with cells prepared using either Lymphoprep™ (Catalog #07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).

This product replaces the EasySep™ Human Eosinophil Enrichment Kit (Catalog #19256) for even faster cell isolations.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Human Eosinophil Isolation Profile

Starting with whole blood prepared using RBC lysis or HetaSep™, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep™, the purities are 2.4% and 96.8% (gated on CD45+), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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English
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17956RF
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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 1
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Safety Data Sheet 2
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17956RF
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English
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Safety Data Sheet 3
Catalog #
17956RF
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All
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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