EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit

Immunomagnetic positive selection of human central and effector memory CD8+ T cells

1 x 10^9 cells
Catalog # 17869
846 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD8+ T cells that are immediately ready for downstream applications. Effector memory CD8+ T cells can be isolated from the CD62L- fraction.
Advantages
• Easy-to-use and column-free
• Up to 93% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
  • EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit (Catalog #17869)
    • EasySep™ Human Memory CD8+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • EasySep™ Human CD62L Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasyeSep™ Release Buffer, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD8+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Catalog #
17869
Lot #
All
Language
English

Educational Materials(7)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Immunomagnetic Purification of Human Central and Effector Memory T Cell Subsets in 45 Minutes

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data


Starting with fresh PBMCs, the central memory CD8+ T cell content (CD3+CD8+CD45RO+CD62L+) of the isolated fraction is typically 86.8 ± 8.4% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD8+CD45RO+CD62L-) of the isolated fraction is typically 88.7 ± 6.5% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

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