EasySep™ Human Basophil Isolation Kit

13-Minute cell isolation kit using immunomagnetic negative selection
Catalog #
17969_C
13-Minute cell isolation kit using immunomagnetic negative selection
From: 740 USD
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Required Products
  1. HetaSep™|07806
    HetaSep™

    For depletion of red blood cells from fresh blood samples and isolation of nucleated cells

  2. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  3. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Basophil Isolation Kit is designed to isolate basophils from a polymorphonuclear cell-rich fraction by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-basophils and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

The isolation kit is compatible with cells prepared using HetaSep™ (Catalog #07906) sedimentation of fresh peripheral blood.

This product replaces the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components
  • EasySep™ Human Basophil Isolation Kit (Catalog #17969)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
  • RoboSep™ Human Basophil Isolation Kit with Filter Tips (Catalog #17969RF)
    • EasySep™ Human Basophil Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Basophil Isolation Kit
Catalog #
17969
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Basophil Isolation Kit
Catalog #
17969RF
Lot #
All
Language
English

Educational Materials(8)

Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
2:10
How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Typical EasySep™ Human Basophil Isolation Profile

Starting with HetaSep™-processsed human peripheral blood, the basophil content (CD45+CD123+IgE+) of the isolated fraction is typically 94.0 ± 2.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 0.86% and 95.6% (gated on CD45+), respectively.

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