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The EasySep™ HLA B Cell Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human B cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD33, CD36, CD41, CD43, CD56, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
• Fast, easy-to-use and column-free
• Up to 99% purity
• Untouched, viable cells
• Compatible with downstream HLA assays
EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA)
EasySep™ HLA B Cell Enrichment Cocktail, 1 mL
EasySep™ Magnetic Particles, 4 x 1 mL
HetaSep™, 20 mL (Catalog #07806) x 2
RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips (Catalog #19954HLARF)
Frequencies of Cell Types in Human Peripheral Blood
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Evaluation of the Robosep Cell Separation for HLA analysis
Cell Enrichment of Specific Lymphocyte Populations (T Cells, B Cells, Total Lymphocytes) from Whole Blood
Frequently Asked Question
Can EasySep™ be used for either positive or negative selection?
Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
How does the separation work?
Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
Which columns do I use?
The EasySep™ procedure is column-free. That's right - no columns!
How can I analyze the purity of my enriched sample?
The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
Can EasySep™ separations be automated?
Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
Can EasySep™ be used to isolate rare cells?
Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.
Are the EasySep™ magnetic particles FACS-compatible?
Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
Can the EasySep™ magnetic particles be removed after enrichment?
No, but due to the small size of these particles, they will not interfere with downstream applications.
Can I alter the separation time in the magnet?
Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
For positive selection, can I perform more than 3 separations to increase purity?
Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Figure 1. FACS Results with EasySep™ HLA B Cell Enrichment Kit
Starting with HetaSep™-treated whole blood, the CD19+ cell content of the enriched fraction typically ranges from 81.5 - 99.7% (gated on CD45+ cells). Starting with 0.5 mL of HetaSep™-treated whole blood (equivalent to 5 mL of whole blood), 1.2 - 9.1 x 10e5 CD19+ cells are typically recovered.
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EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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