CoV Protease Substrate-2 IF670

FRET-based peptide substrate for detection of protease activity of coronaviruses

CoV Protease Substrate-2 IF670

FRET-based peptide substrate for detection of protease activity of coronaviruses

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FRET-based peptide substrate for detection of protease activity of coronaviruses
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Product Advantages


  • Enables efficient detection of coronavirus protease activity

  • Can be used as a high throughput screening tool for inhibitors of coronavirus proteases

Overview

CoV Protease Substrate-2 IF670 allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate can be cleaved by 3C-like proteases (3CLᵖʳᵒ), the CoV main protease involved in regulating the coronavirus replication complex. 3CLᵖʳᵒ is also a potential target for therapeutic applications.

CoV Protease Substrate-2 IF670 has an excitation wavelength of 656 nm, an emission wavelength of 670 nm, an extinction coefficient of 250000 cm⁻¹ M⁻¹. It consists of a 12 amino acid sequence (VNSTLQSGLRKM) that also contains the Tide Quencher™ 5 (TQ5; quencher) and iFluor™ 670 (donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the iFluor™ 670 excited state and the TQ5 absorption leads to efficient intramolecular fluorescence quenching of iFluor™ 670 by the TQ5 fragment. Cleavage of the substrate by CoV proteases, however, dissociates the iFluor™ 670 fragment from TQ5, restoring the fluorescence of the iFluor™ 670 moiety that is directly correlated to the CoV protease activity.
Application
Enzyme Substrate, FRET
Area of Interest
Drug Discovery and Toxicity Testing, Infectious Diseases

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0511, 100-0512
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0511, 100-0512
Lot #
All
Language
English