Animal component-free suspension culture medium for human ES and iPS cells
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Based on TeSR™-E8™, TeSR™-E8™3D is a low-protein medium with an optimized protocol for scaling up human ES and iPS cell production in suspension culture. The novel fed-batch workflow saves time and media; daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.
TeSR™-E8™3D is made with the highest level of quality and care. It contains only the essential components required for scale-up of ES and iPS cells. The TeSR™-E8™3D system is compatible with cells previously cultured in mTeSR™1 or TeSR™-E8™ on Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277) or Vitronectin XF™ (Catalog #07180, a matrix developed and manufactured by Nucleus Biologics), and is compatible with a range of suspension culture vessels.
Figure 1. Human pluripotent stem cell (hPSC) growth in TeSR™-E8™3D
TeSR™-E8™3D supports expansion of hPSCs over multiple passages in aggregate suspension culture. Shown are (A) cumulative fold expansion and (B) daily fold expansion in human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars are +/- SD, n=3.
Figure 2. Viability of hPSCs in TeSR™-E8™3D
hPSCs cultured in TeSR™-E8™3D maintain high viability over multiple passages. Shown are end-of-passage viabilities for human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars are +/- SD, n=3.
Figure 3. Morphology of hPSC aggregates in TeSR™-E8™3D
hPSCs depict morphology characteristic of hPSC aggregates in suspension culture including mostly spherical shape, clear edges (not smooth or shiny), even color, and dimpled or pock-marking appearance. Aggregates should remain under 400 µm in size.
Figure 4. Metabolic Profile of hPSCs during culture in TeSR™-E8™3D
(A) Glucose concentration in TeSR™-E8™3D is maintained at optimal levels throughout the culture period. Dotted line represents the glucose level in TeSR™-E8™3D Complete Seed Medium. Error bars are +/- SD, n = 3, and (B) end-of-passage lactate concentration remains below 15 mM throughout culture in TeSR™-E8™3D. Error bars are +/- SD, n = 3
Figure 5. Markers of undifferentiated hPSCs measured in TeSR™-E8™3D cultures.
Shown are (A) OCT-4 expression and (B) TRA-1-60 expression for multiple hPSC lines after 5 passages in TeSR™-E8™3D. Error bars are +/- SD, n = 3.
Figure 6. Trilineage differentiation of hPSCs cultured in TeSR™-E8™3D.
Trilineage differentiation potential to was assessed using the STEMdiff™ Trilineage Differentiation Kit. hPSCs exhibit equivalent or better differentiation to (A) ectoderm, (B) mesoderm, and (C) endoderm compared to 2D controls, after 5 passages in TeSR™-E8™3D. Error bars are +/- SD, n = 3.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
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TeSR™-E8™3D is manufactured and sold under global exclusive license from Accellta for culture medium for hPSCs in suspension under feeder-free, non-adherent conditions. This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.