TeSR™-E6

Defined, serum-free, xeno-free medium for pluripotent stem cells

TeSR™-E6

Defined, serum-free, xeno-free medium for pluripotent stem cells

From: 251 USD
Catalog #
(Select a product)
Defined, serum-free, xeno-free medium for pluripotent stem cells
Add to Wish List

What's Included

  • TeSR™-E5/E6 Basal Medium, 475 mL
  • TeSR™-E6 20X Supplement, 25 mL

Overview

TeSR™-E6 is a defined, serum- and xeno-free medium that is based on the formulation of TeSR™-E8™, but does not contain transforming growth factor β (TGF-β) or basic fibroblast growth factor (bFGF). It may be used as a basal medium for differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, or other applications where removal of the above cytokines is desirable.
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Characterization, Differentiation
Brand
TeSR
Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology
Formulation Category
Serum-Free, Xeno-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
TeSR™-E6
Catalog #
05946
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
TeSR™-E6
Catalog #
05946
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
TeSR™-E6
Catalog #
05946
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Publications (1)

α-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells. TeSlaa T et al. Cell metabolism 2016 SEP

Abstract

Pluripotent stem cells (PSCs) can self-renew or differentiate from naive or more differentiated, primed, pluripotent states established by specific culture conditions. Increased intracellular α-ketoglutarate (αKG) was shown to favor self-renewal in naive mouse embryonic stem cells (mESCs). The effect of αKG or αKG/succinate levels on differentiation from primed human PSCs (hPSCs) or mouse epiblast stem cells (EpiSCs) remains unknown. We examined primed hPSCs and EpiSCs and show that increased αKG or αKG-to-succinate ratios accelerate, and elevated succinate levels delay, primed PSC differentiation. αKG has been shown to inhibit the mitochondrial ATP synthase and to regulate epigenome-modifying dioxygenase enzymes. Mitochondrial uncoupling did not impede αKG-accelerated primed PSC differentiation. Instead, αKG induced, and succinate impaired, global histone and DNA demethylation in primed PSCs. The data support αKG promotion of self-renewal or differentiation depending on the pluripotent state.