STEMdiff™ Lung Progenitor Kit
Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells
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In-lab demonstration
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Gentle Cell Dissociation ReagentcGMP, enzyme-free cell dissociation reagent
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
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Y-27632 (Dihydrochloride)RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
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mTeSR™1cGMP, feeder-free maintenance medium for human ES and iPS cells
Overview
Data Figures
Figure 1. Generation of Lung Progenitor Cells from Human ES/iPS Cells with the STEMdiff™ Lung Progenitor Kit
Human PSC (hPSC) cultures progress through a simple three-stage process to generate lung progenitor cells. hPSC clumps are first seeded in mTeSR™1. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently on day 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632. Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (12)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
