STEMdiff™ Forebrain Neuron Differentiation Kit

Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

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STEMdiff™ Forebrain Neuron Differentiation Kit

Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

Catalog #08600
255 USD

Required Products

Overview

The STEMdiff™ Forebrain Neuron Differentiation Kit is used in conjunction with the STEMdiff™ Forebrain Neuron Maturation Kit (Catalog #08605) to generate a mixed population of forebrain-type (FOXG1-positive) neurons from neural progenitor cells derived from human pluripotent stem cells. This kit is optimized to work with the STEMdiff™ SMADi Neural Induction Kit that supplies the appropriate neural progenitor cells. Neurons derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.
Advantages:
• Defined and serum-free
• Supports highly efficient generation of functional neurons from hPSC-derived neuronal precursors
• Produces a highly pure population (≥ 90% neurons) of mixed excitatory and inhibitory neurons that can be maintained long-term in culture
• Optimized for differentiation of neuronal progenitor cells generated using the STEMdiff™ SMADi Neural Induction Kit EB Protocol
• Supports neuronal activity for physiologically relevant results
• Enables reproducible maturation of neuronal precursors derived from multiple human ES and iPS cell lines
Components:
  • STEMdiff™ Forebrain Neuron Differentiation Basal Medium
    • STEMdiff™ Forebrain Neuron Differentiation Supplement
Subtype:
Specialized Media
Cell Type:
Neural Cells, PSC-Derived; Neural Stem and Progenitor Cells
Species:
Human
Application:
Cell Culture; Differentiation
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience
Formulation:
Serum-Free; Chemically Defined

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Experimental Protocol Schematic for STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Forebrain-type neural precursors can be generated in 18 - 19 days from hPSC-derived neural progenitor cells (NPCs) after selecting neural rosettes from replated embryoid bodies. For the maturation of precursors to forebrain-type neurons, see the PIS.

Experimental Protocol Schematic for STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 2. Schematic for the Monolayer Protocol

Forebrain-type neural precursors can be generated from neural progenitor cell (NPC) monolayers derived from embryonic and induced pluripotent stem cells after three single-cell passages. For the maturation of precursors to forebrain-type neurons, see the PIS.

Forebrain-Type Neurons Are Generated from Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits

Figure 3. Forebrain-Type Neurons Are Generated After Culture in STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits

NPCs generated from hPSCs in mTeSR 1™ using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to forebrain-type neurons using the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. (A) Forebrain-type neurons were formed after iPS cell-derived NPCs were cultured with the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green), with (C) fewer than 10% astrocytes (GFAP-positive cells, red). (D) Nuclei are labeled with DAPI (blue).

STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits Are Replacing STEMdiff™ Neuron Kits and Have Equivalent Performance in Deriving Forebrain-type Neurons from Neural Progenitor Cells.

Figure 4. Performance of STEMdiff™ Neuron and the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits

Neural progenitor cells (NPCs) were derived using the monolayer protocol for the STEMdiff™ SMADi Neural Induction Kit, from H9 ES cells maintained in mTeSR™ 1. NPCs were then differentiated into forebrain-type neurons using either A) STEMdiff™ Neuron Differentiation and Maturation Kits or B) the equivalent-performing STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits according to protocol instructions (see product information sheets for Catalog #08600/08605). The resulting neurons were fixed for immunofluorescence. Both sets of products demonstrate equivalent differentiation efficiency, with a yield of ≥90% class III ꞵ-tubulin-positive neurons (green) and ≤10% GFAP-positive astrocytes (red).

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.