STEMdiff™ Cardiomyocyte Plating Kit
A specialized media kit for improving plating efficiency post-thawing and post-harvesting of human pluripotent stem cell-derived cardiomyocytes
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STEMdiff™ Cardiomyocyte Maintenance KitMedium for long-term maintenance of human PSC-derived cardiomyocytes
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STEMdiff™ Cardiomyocyte Dissociation KitFor dissociation of hPSC-derived cardiomyocytes
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D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
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Falcon® Serological Pipettes, 10 mLSterile polystyrene pipettes
Overview
This kit is designed for hPSC-derived cardiomyocytes generated using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit. By doubling your cardiomyocyte plating efficiency, the STEMdiff™ Cardiomyocyte Plating Kit can help increase your experimental throughput.
Use this kit as part of a complete, optimized workflow including these compatible STEMdiff™ cardiomyocyte products:
STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
STEMdiff™ Cardiomyocyte Dissociation Kit
STEMdiff™ Cardiomyocyte Support Medium
STEMdiff™ Cardiomyocyte Freezing Medium
STEMdiff™ Cardiomyocyte Maintenance Kit
Data Figures
Figure 1. Increased Plating Efficiency Observed When Using hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit
Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).
Figure 2. Morphology of Replated hPSC-Derived Cardiomyocytes Using STEMdiff™ Cardiomyocyte Plating Kit
Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.
Figure 3. hPSC-Derived Cardiomyocytes Replated Using STEMdiff™ Cardiomyocyte Plating Kit Exhibit a Stable Beat Profile
Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (6)
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
