PneumaCult™ Apical-Out Airway Organoid Medium

Serum- and BPE-free medium for differentiation of human primary bronchial epithelial cells or human airway epithelial cells to mature apical-out airway organoids

PneumaCult™ Apical-Out Airway Organoid Medium

Serum- and BPE-free medium for differentiation of human primary bronchial epithelial cells or human airway epithelial cells to mature apical-out airway organoids

PneumaCult™ Apical-Out Airway Organoid Medium
1 Kit
695 USD
Catalog # 100-0620

Serum- and BPE-free medium for differentiation of human primary bronchial epithelial cells or human airway epithelial cells to mature apical-out airway organoids

PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (24-well plate)
1 Kit
763 USD
Catalog # 100-0746

Serum- and BPE-free medium and AggreWell™400 plate for generation of apical-out airway organoids

PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (5 x 24-well plates)
1 Kit
1,018 USD
Catalog # 100-0747

Serum- and BPE-free medium and AggreWell™400 plates for generation of apical-out airway organoids

Product Advantages

⦁ Convenient, in vitro system that provides easy access to the apical side of the airway epithelium
⦁ Easy downstream processing with a Matrigel®-free protocol
⦁ Generates homogeneous organoids across donors
⦁ Suitable for high-throughput drug screening applications

What's Included

  • PneumaCult™ Apical-Out Airway Organoid Medium, Catalog #100-0620
    • PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
    • PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
    • PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
  • PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (24-well plate), Catalog #100-0746
    • PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
    • PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
    • PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
    • AggreWell™400, 24-well Plate, 1 pack
  • PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (5 x 24-well plates), Catalog #100-0747
    • PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
    • PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
    • PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
    • AggreWell™400, 24-well Plate, 5 pack

Overview

Generate apical-out airway organoids in 15 days from human bronchial epithelial cells (HBECs) or human airway epithelial cells (HAECs) with a simple and scalable, Matrigel®-free protocol. These organoids provide access to the apical side of the airway epithelium and can be used to perform your infectious disease modeling or high-throughput drug screening in an in vitro, three-dimensional (3D) model system.

With PneumaCult™ Apical-Out Airway Organoid Medium, you can grow polarized airway organoids with outward-facing, differentiated ciliated cells in AggreWell™400 24-well plates (Catalog #34415)—without the use of cell culture inserts. To maximize experimental reproducibility, this medium is formulated without serum or BPE. For a complete apical-out airway organoid culture workflow, expand your HBECs or HAECs in PneumaCult™-Ex Plus Medium (Catalog #05040) prior to initiating organoid culture with PneumaCult™ Apical-Out Airway Organoid Medium.

Find more about the applications of lung organoids in our learning center.
Subtype
Specialized Media
Cell Type
Airway Cells, Epithelial Cells
Species
Human
Application
Cell Culture, Differentiation, Organoid Culture
Brand
PneumaCult
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Infectious Diseases, Organoids, Respiratory Research, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0620
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0620
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0620
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0620
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0746
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0746
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0746
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Schematic showing the workflow for generation of apical-out airway organoids using PneumaCult™.

Figure 1. Generation of Apical-Out Airway Organoids Using the PneumaCult™ Apical-Out Airway Organoid Medium

In the two-dimensional expansion phase of the human apical-out airway organoid culture protocol, HBECs or HAECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs or HAECs are then plated into an AggreWell™400 24-well plate and allowed to aggregate for 1 - 6 days using PneumaCult™ Apical-Out Airway Organoid Medium. Following the aggregation, the cells are dislodged from the microwells and the aggregate suspension is transfered to a 24-well flat-bottom plate and differentiated for 9 - 14 days using PneumaCult™ Apical-Out Airway Organoid Medium to generate apical-out airway organoids that display beating cilia.

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