PancreaCult™ Organoid Media (Human)

Culture media kits for initiation, growth, and establishment of human pancreatic organoids

PancreaCult™ Organoid Media (Human)

Culture media kits for initiation, growth, and establishment of human pancreatic organoids

From: 589 USD
Catalog #
100-0820_C
Culture media kits for initiation, growth, and establishment of human pancreatic organoids

Product Advantages

• Convenient, in vitro system that recapitulates many key characteristics of adult pancreatic ducts
• Enables efficient organoid initiation and expansion across different pancreatic donor tissues
• Flexible growth medium allow expansion of normal and tumor-derived organoids
• Serum-free and conditioned medium-free

What's Included

  • PancreaCult™ Organoid Initiation Medium (Human) (Catalog #100-0820)
    • PancreaCult™ Organoid Basal Medium (Human) , 95 mL
    • PancreaCult™ Organoid Growth Supplement (Human), 5 mL
    • Organoid Supplement, 50 mL
  • PancreaCult™ Organoid Growth Medium (Human) (Catalog #100-0781)
    • PancreaCult™ Organoid Basal Medium (Human) , 95 mL
    • PancreaCult™ Organoid Growth Supplement (Human), 5 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Product Information Sheet.

Overview

Reliably culture pancreatic duct organoids to study the function of the ductal epithelium, model pancreatic diseases such as cancer or cystic fibrosis, and perform targeted drug screens or toxicity assays. With the PancreaCult™ (Human) culture medium system, you have a complete and robust workflow to establish, expand, and maintain pancreatic duct organoids.

Pancreatic organoids are established from fresh or cryopreserved islet-depleted exocrine fractions in PancreaCult™ Organoid Initiation Medium (OIM), which is optimized to provide higher rates of organoid formation from tissue. These organoids are then transferred to PancreaCult™ Organoid Growth Medium (OGM) for long-term maintenance and passaging, or cryopreservation for future experiments. Organoids grown using the PancreaCult™ (Human) medium system can be adapted to various culture protocols, including two-dimensional Transwell® monolayer cultures, dilute Corning® Matrigel® suspension cultures, and high-throughput assay-compatible culture formats.
Subtype
Specialized Media
Cell Type
Pancreatic Cells
Species
Human
Application
Cell Culture, Expansion, Maintenance, Organoid Culture
Brand
PancreaCult
Area of Interest
Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0820
Lot #
All
Language
English
Catalog #
100-0781
Lot #
All
Language
English
Document Type
Technical Manual
Catalog #
100-0820
Lot #
All
Language
English
Document Type
Technical Manual
Catalog #
100-0781
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0820
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0820
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0820
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0781
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0781
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids

Data and Publications

Human Pancreatic Duct Organoids Grown using PancreaCult™ (Human)

Figure 1. Human Pancreatic Duct Organoids

PancreaCult™ Organoid Initiation Medium (OIM; Human) and Organoid Growth Medium (OGM; Human) support the initiation and expansion of human pancreatic duct organoids from pancreatic tissue or previously established organoid cultures. Shown are organoids grown using PancreaCult™ OIM and OGM and imaged on day 7 of passage 3.

Workflow for Generating Pancreatic Ductal Organoids Using PancreaCult™ (Human)

Figure 2. PancreaCult™ Human Enables Initiation and Expansion of Pancreatic Ductal Organoids

Human pancreatic ductal organoids are initiated in PancreaCult™ OIM for the first 3 days of culture, then switched to PancreaCult™ OGM for the remainder of culture. Alternatively, organoids can be grown in PancreaCult™ OGM for a completely serum-free protocol, however, proliferative pancreatic ductal cells are better supported in PancreaCult™ OIM during the first 3 days of culture. Cultures should be passaged after 7 days with further medium changes every 2-3 days. Pancreatic ductal organoids are suitable for experimentation or banking after one full passage in PancreaCult™ OGM. For full culture instructions please refer to the product manual (Document #10000011617)

PancreaCult™ (Human) Provides Robust Expansion of Pancreatic Duct Organoids

Figure 3. PancreaCult™ (Human) Provides Robust Expansion of Pancreatic Duct Organoids

(A) Organoid expansion in PancreaCult™ OGM provides robust expansion of organoids across different donors. (B) Organoids may be initiated in PancreaCult™ OGM for a completely serum-free workflow, however, initiation and maintenance of damaged tissue is better supported by initiating cultures in PancreaCult™ OIM. (C) Comparison of PancreaCult™ Human to two different DIY formulations showed more robust expansion of organoids in PancreaCult™ Human. Shown is the cumulative fold expansion of organoid fragments as counted at the end of each passage.

Pancreatic Duct Organoids Display Features of the Pancreatic Ductal Epithelium by ICC Staining

Figure 4. Pancreatic Duct Organoids Display Features of the Pancreatic Ductal Epithelium

Organoids grown using the PancreaCult™ (Human) display marker expression consistent with the pancreatic ductal epithelium when imaged using immunocytochemistry. Shown are organoids grown in PancreaCult™ OGM and stained for (A) pancreatic ductal marker CK19, (B) pancreatic ductal marker SOX9, (C) epithelial marker EPCAM, (D) proliferation marker KI67, (E) apical pancreatic duct marker MUC1, and (F) pancreatic ductal marker CA2. Organoids were imaged on passage 2 (A), passage 3 (B, C) or passage 10 (D-F).

Pancreatic Duct Organoids Cultured With PancreaCult™ Human Show Pancreatic Marker Expression Levels Similar to Exocrine Tissue

Figure 5. Pancreatic Duct Organoids Cultured With PancreaCult™ Human Show Pancreatic Marker Expression Levels Similar to Exocrine Tissue

Pancreatic duct organoids grown using the PancreaCult™ Human show marker expression levels similar to those observed in exocrine tissue. Analysis by qPCR showed pancreatic duct organoids were enriched for (A) PDX1 (C) CK19 and (F) LGR5 as compared to total pancreatic tissue, demonstrating enrichment of proliferative duct organoids. Comparable expression of (B) SOX9, (D) cystic fibrosis transmembrane receptor (CFTR), and (E) CA2 was observed in pancreatic duct organoids. Expression levels are normalized to TBP and UBC housekeeping genes (ΔCT) and total pancreas for relative expression levels (ΔΔCT).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.