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MesenCult™-ACF Plus Umbilical Cord Culture Kit

Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue

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MesenCult™-ACF Plus Umbilical Cord Culture Kit

Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue

From: 759 USD
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Animal component-free medium for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue
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Product Advantages


  • Supports high yield of MSCs from umbilical cord tissue

  • Umbilical cord-derived MSCs consistently express MSC-specific markers and retain superior expansion and differentiation capabilities

  • Complete animal component-free workflow compatible with MesenCult™-ACF portfolio

What's Included

  • MesenCult™-ACF Plus Medium, 500 mL
  • MesenCult™ Umbilical Cord Derivation 500X Supplement, 0.5 mL
  • MesenCult™-ACF Plus 500X Supplement, 0.5 mL
  • Animal Component-Free Cell Attachment Substrate, 3 x 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  2. L-Glutamine
    L-Glutamine

    Cell culture supplement

  3. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs.

MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.

NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials.

CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.

The Product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.
Subtype
Specialized Media
Cell Type
Mesenchymal Stromal Cells
Species
Human
Application
Cell Culture, Cell Isolation
Brand
MesenCult
Area of Interest
Cell Line Development, Stem Cell Biology
Formulation Category
Animal Component-Free

Data Figures

Figure 1. Workflow for Expanding UC-MSCs Using the MesenCult™-ACF Plus Umbilical Cord Culture Kit

Protocol for deriving and expanding umbilical cord-derived mesenchymal stromal cells (UC-MSCs) with the MesenCult™-ACF Plus Umbilical Cord Culture Kit. UC-MSCs are derived in 14 days using MesenCult™-ACF Plus Medium supplemented with the MesenCult™-ACF Plus Umbilical Cord Derivation 500X Supplement. After Day 14, cells can be maintained in complete MesenCult™-ACF Plus Medium. Representative images at Day 6, 14, and 21 are included for reference. Click here for the full protocol.

Figure 2. High UC-MSC Yield Is Observed When Isolated with MesenCult™-ACF Plus Umbilical Cord Culture Kit

Compared to MesenCult™-ACF Plus (MACFP) medium, the isolation yield, per gram umbilical cord tissue, of UC-MSCs in MesenCult™-ACF Plus Umbilical Cord (MACFP-UC) Culture Kit is 65% higher. The addition of 2.5% human AB serum during isolation further increases the yield to over 4 times more than with MesenCult™-ACF Plus alone.

Figure 3. Consistent Growth and Morphology for over 20 Passages of UC-MSCs Cultured with the MesenCult™-ACF Umbilical Cord Culture Kit

(A) MesenCult™-ACF Plus Umbilical Cord Culture Kit supports the long-term expansion of UC-MSCs. UC-MSCs isolated and expanded according to the MesenCult™-ACF Plus Umbilical Cord Culture Kit protocol can be cultured for up to 23 passages. (B) The morphology of UC-MSCs from two donors remains MSC-like following 21 passages in MesenCult™-ACF Plus Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
MesenCult™-ACF Plus Umbilical Cord Culture Kit
Catalog #
100-0234
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (7)

Products for Your Mesenchymal Stromal Cell Research
Brochure
Products for Your Mesenchymal Stromal Cell Research
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
Technical Bulletin
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus
The Identity and Properties of Mesenchymal Stem Cells
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Wallchart
Mesenchymal Stromal Cell-Derived Exosomes: Biogenesis and Cargoes
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
13:45
Video
Isolation of Umbilical Cord Mesenchymal Stromal Cells Using Explant Method
The Secret In Vivo Life of "Mesenchymal Stem Cells"
58:00
Webinar
The Secret In Vivo Life of "Mesenchymal Stem Cells"
Tools to Accelerate Your MSC and EV Workflows
30:05
Webinar
Tools to Accelerate Your MSC and EV Workflows

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Quality Statement:

STEMCELL TECHNOLOGIES INC.'S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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