MesenCult™-ACF Culture Kit

Defined, animal component-free culture kit for human mesenchymal stem cells

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MesenCult™-ACF Culture Kit

Defined, animal component-free culture kit for human mesenchymal stem cells

1 Kit
Catalog #05449
391 USD

Required Products

Overview

MesenCult™-ACF Culture Kit is a standardized, animal component-free and serum-free system for the isolation and culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-ACF Medium (Catalog #05440) and MesenCult™-ACF Attachment Substrate (Catalog #05444) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit-fibroblast (CFU-F) assay. This product supports the long-term growth of human bone marrow- and adipose tissue-derived MSCs as cells maintain robust multi-lineage differentiation potential. MesenCult™-ACF Medium must be used in conjunction with MesenCult™-ACF Attachment Substrate and MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of MesenCult™-ACF Attachment Substrate and MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Medium. MesenCult™-ACF Medium must be supplemented with L-glutamine.
Advantages:
• Defined, animal component-free culture system
• Optimized for MSC isolation from primary bone marrow and adipose tissue
• Superior cell expansion compared to serum-containing medium
• MSC cultures display robust self-renewal and differentiation potential after long-term passaging
• No adaptation required when transitioning MSCs from serum-containing medium
Components:
  • MesenCult™-ACF Culture Kit (Catalog #05449)
    • MesenCult™-ACF Basal Medium, 400 mL (Catalog #05451)
    • MesenCult™-ACF 5X Supplement, 100 mL (Catalog #05452)
    • MesenCult™-ACF Attachment Substrate, 1 mL (Catalog #05444)
Subtype:
Specialized Media
Cell Type:
Mesenchymal Stem and Progenitor Cells
Species:
Human
Application:
Cell Culture; Colony Assay; Expansion
Brand:
MesenCult
Area of Interest:
Stem Cell Biology
Formulation:
Animal Component-Free; Serum-Free; Defined

Technical Resources

Educational Materials

(10)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Human BM-derived MSCs Cultured in MesenCult™-ACF Display Multi-lineage Differentiation Potential

Figure 1. Human BM-derived MSCs Cultured in MesenCult™-ACF Display Multi-lineage Differentiation Potential

Human BM-derived MSCs cultured in MesenCult™-ACF Medium for 3 passages (A) differentiate into adipocytes (B, Oil Red O staining), osteogenic cells (C, Von Kossa/ALP staining) and chondrocytes (D, Alcian Blue + Nuclear Fast Red staining).

Human BM-derived MSCs Cultured in MesenCult™-ACF Expand Faster Than Cells Cultured in a Traditional Serum-based Medium or Competitor Media

Figure 2. Human BM-derived MSCs Cultured in MesenCult™-ACF Expand Faster Than Cells Cultured in a Traditional Serum-based Medium or Competitor Media

(n = 3 except for Competitor 3 where n = 2, Mean ± SEM).

Human BM-derived MSCs Cultured in MesenCult™-ACF Display Characteristic Expression of MSC Surface Markers

Figure 3. Human BM-derived MSCs Cultured in MesenCult™-ACF Display Characteristic Expression of MSC Surface Markers

Human BM-derived MSCs cultured in MesenCult™-ACF were stained at Passage 4 with antibodies to mesenchymal markers (CD90, CD73 and CD105) and hematopoietic markers (CD45, CD11b and HLA-DR).

Publications

(1)
Cytotherapy 2006 JAN

Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.

Dominici M et al.

Abstract

The considerable therapeutic potential of human multipotent mesenchymal stromal cells (MSC) has generated markedly increasing interest in a wide variety of biomedical disciplines. However, investigators report studies of MSC using different methods of isolation and expansion, and different approaches to characterizing the cells. Thus it is increasingly difficult to compare and contrast study outcomes, which hinders progress in the field. To begin to address this issue, the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC. First, MSC must be plastic-adherent when maintained in standard culture conditions. Second, MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19 and HLA-DR surface molecules. Third, MSC must differentiate to osteoblasts, adipocytes and chondroblasts in vitro. While these criteria will probably require modification as new knowledge unfolds, we believe this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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