ImmunoCult™ Human Th1 Differentiation Supplement

Serum-free culture supplement for the differentiation of human naïve CD4+ T cells into Th1 cells

ImmunoCult™ Human Th1 Differentiation Supplement

Serum-free culture supplement for the differentiation of human naïve CD4+ T cells into Th1 cells

From: 362 USD
Catalog #
10973_C
Serum-free culture supplement for the differentiation of human naïve CD4+ T cells into Th1 cells

Product Advantages


  • Formulated for the induction of human Th1 differentiation

  • Optimized for use with ImmunoCult™-XF T Cell Expansion Medium

  • Supports significantly higher levels of memory T cells in comparison to competitor Th1 differentiation kit

  • Supports up to 1.6X higher expansion of IFN-γ-producing CD4+ T cells in comparison to competitor Th1 differentiation kit

  • Supplied as a 50X concentrate; after thawing and mixing, the tube contents can be added directly to medium

Overview

ImmunoCult™ Human Th1 Differentiation Supplement contains a combination of human cytokines and a blocking antibody formulated to promote the robust activation, expansion, and differentiation of peripheral blood-derived, naïve, CD4+ human T cells into Th1 cells. ImmunoCult™ Human Th1 Differentiation Supplement is intended for use with ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) and ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971).

This product is recommended for:
• Research into the regulation of human naïve T cell differentiation and Th1 function
• Development of procedures to expand and polarize naïve T cells toward a Th1 subset
Contains
• Recombinant human interleukin 12 (IL-12)
• Recombinant human interleukin 18 (IL-18)
• Mouse anti-human interleukin 4 (anti-IL-4)
Subtype
Supplements
Cell Type
T Cells, T Cells, CD4+
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
ImmunoCult
Area of Interest
Immunology

Data Figures

Figure 1. ImmunoCult™ Human Th1 Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Th1 Differentiation Supplement Produces More Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973) in ImmunoCult™ and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype was significantly lower and the percentage of cells expressing a differentiated phenotype was significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p < 0.05; paired t-test on logit transformed data; n = 4).

Figure 3. Culture with ImmunoCult™ Human Th1 Differentiation Supplement Produces More IFNγ-Expressing Cells Than Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. After culture, differentiated CD4+ T cells (CD45RA-CD45RO+) were analyzed for IFNγ and IL-4 (not shown) expression, following stimulation with PMA and Ionomycin for 4 hours. A greater percentage of cells generated with ImmunoCult™ expressed intracellular IFNγ compared to those generated under competitor conditions, while no significant difference was found in the percentage of cells which expressed intracellular IL-4, with very low numbers of cells expressing IL-4 for both ImmunoCult™ and competitor. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p< 0.05; paired t-test on logit transformed data; n = 4).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
10973
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
10973
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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