ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator

Human T cell activation and expansion reagent

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From: 131 CAD

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Human T cell activation and expansion reagent
From: 131 CAD

Overview

ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator is designed to activate and expand human T cells in the absence of magnetic beads, feeder cells or antigen. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble tetrameric antibody complexes that bind CD3, CD28 and CD2 cell surface ligands. Binding of the tetrameric antibody complexes results in the cross-linking of CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. Activated T cells can be expanded in ImmunoCult™-XF T Cell Expansion Medium or other media for culturing human T cells supplemented with cytokines.

This product aims to be developed as a cGMP-grade reagent for cellular therapy research. Learn more at www.stemcell.com/t-cell-therapy
Advantages:
• Robust activation and expansion of human T cells without the use of magnetic beads, feeder cells, or antigen
• Provides a gentle activation stimulus that maintains high viability of activated and expanded T cells
• Highly stable, filter-sterilized soluble reagent
Contains:
• Anti-human CD3 monospecific tetrameric antibody complex
• Anti-human CD28 monospecific tetrameric antibody complex
• Anti-human CD2 monospecific tetrameric antibody complex
Subtype:
Supplements
Cell Type:
T Cells; T Cells, CD4+; T Cells, CD8+
Species:
Human
Application:
Activation; Expansion; Cell Culture
Brand:
ImmunoCult
Area of Interest:
Immunology; T Cell Engineering

Technical Resources

Educational Materials

(11)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Activated Morphology of Human T Cells Stimulated With ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator

Figure 1. Activated Morphology of Human T Cells Stimulated With ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator

Image of human T cells isolated using the EasySep™ Human T Cell Isolation Kit (Catalog #17951), stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, and cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981).

Activation of EasySep™ Isolated Human T Cells Stimulated With ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator

Figure 2. Activation of EasySep™ Isolated Human T Cells Stimulated With ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator

EasySep™-isolated human T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator and cultured in ImmunoCult™-XF T Cell Expansion Medium. Activation of viable CD3+ T cells was assessed by CD25 expression using flow cytometry. On day 0, the frequency of CD25 positive cells was (A) 5.6 ± 2.4% (mean ± SD). Following 3 days of culture, the frequency of CD25 positive cells was (B) 88.8 ± 3.2% (mean ± SD) when stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator.

Publications

(1)
Blood 2016 JUL

LFA-1 integrin antibodies inhibit leukocyte $4$1-mediated adhesion by intracellular signaling.

Grönholm M et al.

Abstract

Binding of ICAM-1 (intercellular adhesion molecule-1) to the $2-integrin LFA-1 (leukocyte function associated antigen-1) is known to induce crosstalk to the $4$1 integrin. Using different LFA-1 monoclonal antibodies we have been able to study the requirement and mechanism of action for the crosstalk in considerable detail. LFA-1 activating antibodies and those inhibitory antibodies that signal to $4$1 induce phosphorylation of Thr-758 on the $2-chain, which is followed by binding of 14-3-3 proteins and signaling through the G protein exchange factor Tiam1. This results in dephosphorylation of Thr-788/789 on the $1-chain of $4$1 and loss of binding to its ligand VCAM-1 (vascular cell adhesion molecule-1). The results show that with LFA-1 antibodies, we can either 1) activate LFA-1 and inhibit $4$1, 2) inhibit both LFA-1 and $4$1, 3) inhibit LFA-1 but not $4$1 or 4) not affect LFA-1 or $4$1 These findings are important for the understanding of integrin regulation and for the interpretation of the effect of integrin antibodies and their use in clinical applications.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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