Agar Leukocyte Conditioned Medium
Source of colony-stimulating factors for assays of human hematopoietic progenitor cells
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Agar Leukocyte Conditioned Medium -
Label for Agar Leukocyte Conditioned Medium
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Overview
Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. It is suitable for the growth of colony-forming unit erythroid (CFUE), burst-forming unit-erythroid (BFU-E), colony-forming unit-macrophage (CFU-M), colony-forming unit-granulocyte (CFU-G), colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM) when added to MethoCult™ medium at a final concentration of 10% with the addition of erythropoietin (EPO; Catalog #02625).
Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
Contains:
• Agar Leukocyte Conditioned Medium
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
Subtype:
Basal Media
Cell Type:
Hematopoietic Stem and Progenitor Cells
Species:
Human
Application:
Cell Culture
Area of Interest:
Stem Cell Biology
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Methylcellulose-based medium with EPO for human cells
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.