Agar Leukocyte Conditioned Medium
Source of colony-stimulating factors for assays of human hematopoietic progenitor cells
Overview
Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. It is suitable for the growth of colony-forming unit erythroid (CFUE), burst-forming unit-erythroid (BFU-E), colony-forming unit-macrophage (CFU-M), colony-forming unit-granulocyte (CFU-G), colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM) when added to MethoCult™ medium at a final concentration of 10% with the addition of erythropoietin (EPO; Catalog #02625).
Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
Contains
• Agar Leukocyte Conditioned Medium
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
Subtype
Basal Media
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Application
Cell Culture
Area of Interest
Stem Cell Biology
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Agar Leukocyte Conditioned Medium
Catalog #
02300 Lot #
All Language
English Document Type
Safety Data Sheet
Product Name
Agar Leukocyte Conditioned Medium
Catalog #
02300 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Hematopoietic Stem and Progenitor Cell Research
Resources and Publications
Educational Materials (1)
Related Products
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
