It stimulates the growth of erythroid progenitors (BFU-E and CFU-E), granulocyte-macrophage progenitors (CFU-GM, CFU-G and CFU-M) and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitors (CFU-GEMM) when added to methylcellulose at a final concentration of 10% with the addition of erythropoietin.
Agar Leukocyte Conditioned Medium is prepared using normal human peripheral blood leukocytes in an agar-containing medium.
• Fetal bovine serum (10%)
• Iscove's MDM
• 1 x 10^4 2-Mercaptoethanol (ME)
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