Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071

Rat monoclonal IgG1, kappa isotype control antibody

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Rat monoclonal IgG1, kappa isotype control antibody
From: 36 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The RTK2071 antibody (IgG1, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype:
Isotype Controls
Target Antigen:
IgG1 Isotype Control
Alternative Names:
Not applicable
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated
Host Species:
Rat
Cell Type:
Other
Application:
Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
RTK2071
Gene ID:
Not applicable
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Complete Antibody Listing
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Hybridoma Production
Pre-Enrichment and Fusion
Selection and Cloning
Expansion and Characterization

Data and Publications

Data

Data for unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.