Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
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(2)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 3. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 4. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.