Anti-Phospho-SHIP Antibody, Polyclonal

Rabbit polyclonal antibody against human, mouse phosphoSHIP

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Rabbit polyclonal antibody against human, mouse phosphoSHIP
468 USD

Overview

Phospho-SHIP is a highly phosphorylated form of SHIP. SH2-containing inositol phosphatase (SHIP) is a 145 kDa hematopoietic-restricted protein that becomes tyrosine-phosphorylated and associated with the adaptor protein, Shc, following cytokine, growth factor, chemokine, and immunoreceptor stimulation. SHIP also hydrolyzes the critical phosphatidylinositol (PI)-3-kinase (PI3K)-generated second messenger, PI-3,4,5-P3 (PIP3), to PI-3,4-P2 1,2 and therefore acts as an important negative regulator of the PI3K pathway. This antibody reacts with mouse and human tyrosine-phosphorylated SHIP.
Subtype:
Primary Antibodies
Target Antigen:
PhosphoSHIP
Alternative Names:
p150Ship; phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SHIP-1; SHIP1; SIP-145; s-SHIP
Reactive Species:
Mouse; Human
Conjugation:
Unconjugated
Host Species:
Rabbit
Cell Type:
Granulocytes and Subsets; Lymphocytes; Myeloid Cells
Application:
Immunoblotting; Immunoprecipitation; Flow Cytometry; Immunofluorescence
Area of Interest:
Immunology
Clone:
Not applicable
Gene ID:
3635 (human), 16331 (mouse)
Isotype:
Not applicable

Scientific Resources

Educational Materials

(2)

Data and Publications

Data

(A) Flow cytometry analysis of bone marrow derived mast cells (BMMCs) from normal (+/+) or SHIP knockout (-/-) mice. Cells were stimulated with stem cell factor (SCF; filled histogram) or left unstimulated (solid line historgram), and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(B) BMMCs were either stimulated with SCF or left unstimulated, and then fixed, permeabilized, and labeled with Anti-Phospho-SHIP Antibody, Polyclonal, followed by an anti-rabbit Alexa Fluor® 488.

(C) Western blot analysis of total cell lysates from human TF-1 cells stimulated with IL-3, mouse WEHI-231 B cells stimulated with anti-IgM, and mouse BMMCs stimulated with SCF, and probed with Anti-Phospho-SHIP Antibody, Polyclonal. The blot was re-probed with an anti-SHIP antibody to show equal loading of unstimulated (C) and stimulated (S) samples. Phospho-SHIP has a predicted molecular mass of 145 kDa.

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