Anti-Mouse Sca1 Antibody, Clone E13-161.7

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E)

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Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E)
From: 78 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The E13-161.7 antibody reacts with Sca1 (Stem Cell Antigen-1 or Ly-6A/E), an 18 kDa GPI-linked protein belonging to the lymphocyte activation protein-6 (Ly-6) family. Sca1 is expressed on the surface of hematopoietic stem and progenitor cells, myeloid cells, and peripheral B and T lymphocytes. Sca1 is expressed by mice with either the Ly-6.1 or Ly-6.2 allotypes but the pattern of expression differs in the circulating cell population according to the allotype. Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) possess relatively high numbers of Sca1+ resting lymphocytes compared to Ly-6.1 strains (e.g. A, BALB/c, CBA, C3H/He, DBA/1, NZB). Sca1 expression levels are strongly upregulated in all strains upon cellular activation. Sca1 is involved in the regulation of T and B cell responses and is believed to play roles in the differentiation, proliferation, and survival of a variety of stem cells. Sca1 has emerged as a phenotypic marker of choice for identifying and isolating hematopoietic stem and progenitor cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse SCA1 Biotin Positive Selection Kit (Catalog #18856).
Subtype:
Primary Antibodies
Target Antigen:
Sca1 (Ly-6A/E)
Alternative Names:
Ly-6A/E, Sca-1
Reactive Species:
Mouse
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated
Host Species:
Rat
Cell Type:
Hematopoietic Stem and Progenitor Cells; Mammary Cells
Application:
Cell Isolation; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation
Area of Interest:
Stem Cell Biology; Epithelial Cell Biology
Clone:
E13-161.7
Gene ID:
110454
Isotype:
IgG2a, kappa

Scientific Resources

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(4)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
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Complete Antibody Listing
Brochure
Tools For Your Immunology Research
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Cell Culture Antibodies

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 and processed with the EasySep™ Mouse SCA1 Positive Selection Kit. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ PE Selection Kit for Mouse Cells (Catalog #18554) using Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.