Anti-Mouse OCT4 (OCT3) Antibody, Clone 40
Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)
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Anti-Mouse OCT4 (OCT3) Antibody, Clone 40 -
Label for Anti-Mouse OCT4 (OCT3) Antibody, Clone 40
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Overview
The 40 antibody clone reacts with OCT-3/4, also known as POU5F1, a transcription factor that is essential for the formation of the inner cell mass during mouse development and for the maintenance of undifferentiated mouse and human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is expressed at high levels in undifferentiated mouse and human ES and iPS cells and in embryonic germ cells (EGCs), and exhibits decreased expression during differentiation. It is commonly used as a marker for the assessment of undifferentiated ES and iPS cells, and EGCs from multiple species.
Subtype:
Primary Antibodies
Target Antigen:
OCT4 (OCT3)
Alternative Names:
Oct3, OTF3, Oct4, OTF4, POU5F1
Reactive Species:
Mouse; Human
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Pluripotent Stem Cells
Application:
Flow Cytometry; Immunofluorescence; Western Blotting
Area of Interest:
Stem Cell Biology
Clone:
40
Gene ID:
18999 (mouse), 5460 (human)
Isotype:
IgG1, kappa
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by a goat antimouse IgG antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). (B) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1 isotype control antibody, followed by goat anti-mouse IgG, FITC.
(C) H1 cell nuclei stained with DAPI. (D) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.