Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3)

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Anti-Mouse OCT4 (OCT3) Antibody, Clone 40

Mouse monoclonal IgG1 antibody against human, mouse OCT4 (OCT3), unconjugated

50 µg
Catalog #60059
670 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The 40 antibody clone reacts with OCT-3/4, also known as POU5F1, a transcription factor that is essential for the formation of the inner cell mass during mouse development and for the maintenance of undifferentiated mouse and human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is expressed at high levels in undifferentiated mouse and human ES and iPS cells and in embryonic germ cells (EGCs), and exhibits decreased expression during differentiation. It is commonly used as a marker for the assessment of undifferentiated ES and iPS cells, and EGCs from multiple species.
Subtype:
Primary Antibodies
Target Antigen:
OCT4 (OCT3)
Alternative Names:
Oct3, OTF3, Oct4, OTF4, POU5F1
Reactive Species:
Mouse; Human
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Pluripotent Stem Cells
Application:
Flow Cytometry; Immunofluorescence; Western Blotting
Area of Interest:
Stem Cell Biology
Clone:
40
Gene ID:
18999 (mouse), 5460 (human)
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Complete Antibody Listing
Brochure
Products for Human Pluripotent Stem Cells
Brochure
Cell Culture Antibodies

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
hPSC-Derived Endoderm Cell Research
Endoderm Induction
Characterization and Isolation
Downstream Differentiation
hPSC-Derived Mesoderm Cell Research
Mesoderm Induction
Downstream Differentiation
PSC Quality Control
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Data and Publications

Data

(A) Flow cytometry analysis of human embryonic stem (ES) cells labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by a goat antimouse IgG antibody, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG antibody, FITC (solid line histogram). (B) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1 isotype control antibody, followed by goat anti-mouse IgG, FITC.

Neurospheres derived from human fetal CNS tissue can be efficiently generated for multiple passages in NeuroCult™-ACF Proliferation Medium

(C) H1 cell nuclei stained with DAPI. (D) H1 cells were labeled with Anti-Mouse OCT4 (OCT3) Antibody, Clone 40, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.