Anti-Mouse Ly-6G Antibody, Clone 1A8

Rat monoclonal IgG2a antibody against mouse Ly-6G

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Rat monoclonal IgG2a antibody against mouse Ly-6G
From: 104 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).
Subtype:
Primary Antibodies
Target Antigen:
Ly-6G
Alternative Names:
Gr-1, GR1, Ly6G
Reactive Species:
Mouse
Conjugation:
Pacific Blue; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated
Host Species:
Rat
Cell Type:
Granulocytes and Subsets; Myeloid Cells
Application:
Flow Cytometry; Functional Assay; Immunocytochemistry; Immunoprecipitation
Area of Interest:
Immunology
Clone:
1A8
Gene ID:
546644
Isotype:
IgG2a, kappa

Scientific Resources

Product Documentation

Educational Materials

(4)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Dendritic Cell Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Pacific Blue™ is a trademark of Life Technologies Corporation. Antibodies conjugated to Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.