Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14

Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin)
Catalog #
60109_C
Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin)
From: 63 USD

Overview

The MEL-14 antibody reacts with murine CD62L (L-Selectin or LECAM-1), an ~74 - 95 kDa type I glycoprotein expressed on the surface of a majority of leukocytes. CD62L mediates leukocyte-endothelial cell interactions through its association with ligands such as CD34, glyCAM-1, MAdCAM-1 and PSGL-1. It functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to the lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. Murine CD62L is expressed on most thymocytes, the highest expression levels being found on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T and NK cells. The MEL-14 antibody may be used together with antibodies to other cell surface markers to distinguish naïve, memory and effector T cells. The MEL-14 antibody has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling.
Subtype
Primary Antibodies
Target Antigen
CD62L (L-selectin)
Alternative Names
CD62 ligand, L-selectin, LAM-1, LECAM-1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Ly-22, MEL-14, Pln homing receptor, PLNHR
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
T Cells
Species
Mouse
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
MEL-14
Gene ID
20343
Isotype
IgG2a, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14
Catalog #
60109, 60109.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488
Catalog #
60109AD, 60109AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC
Catalog #
60109AZ, 60109AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin
Catalog #
60109BT, 60109BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC
Catalog #
60109FI, 60109FI.1, 60109FI.2
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE
Catalog #
60109PE, 60109PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5
Catalog #
60109PS, 60109PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14
Catalog #
60109, 60109.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488
Catalog #
60109AD, 60109AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC
Catalog #
60109AZ, 60109AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin
Catalog #
60109BT, 60109BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC
Catalog #
60109FI, 60109FI.1, 60109FI.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE
Catalog #
60109PE, 60109PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5
Catalog #
60109PS, 60109PS.1
Lot #
All
Language
English

Educational Materials (3)

Brochure
Reliable Antibodies for Your Research
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow Cytometry Analysis of C57BL/6 Mouse Splenocytes

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of mouse splenocytes (gated on CD4+ cells) processed with EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit and labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE and an anti-mouse CD44 antibody, FITC. Labeling of the Start splenocytes prior to cell isolation is shown (C).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.