Anti-Human Tight Junction Protein 1 (ZO-1) Antibody, Polyclonal

Rabbit polyclonal IgG antibody against human tight junction protein 1 (ZO-1)
Catalog #
100-0750_C
Rabbit polyclonal IgG antibody against human tight junction protein 1 (ZO-1)
From: 219 USD

Overview

The anti-human tight junction protein 1 (TJP1) antibody reacts with human TJP1, also known as the zona occludens-1 (ZO-1) protein. TJP1 is a 220 kDa membrane-associated signaling protein that belongs to the family of membrane-associated guanylate kinase (MAGUK) homologs. The TJP1 protein contains three postsynaptic density, disc-large, ZO-1 domains (PDZ), a single Src Homology-3 domain (SH3), and a catalytically inactive guanylate kinase (GK) domain. TJP1 shares ~65% overall similarity with ZO-2 and ZO-3 proteins, with the highest levels of similarity in the MAGUK and acid domains. The TJP1 proteins, along with other proteins such as occludin and claudins, provide anchorage between strand proteins and the actin cytoskeleton. TJP1 dysfunction has been associated with cancer metastasis due to the loss of tight junction barrier function.
Target Antigen
Tight Junction Protein 1
Alternative Names
Zonula Occludens-1, ZO-1
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Rabbit
Cell Type
Airway Cells, Epithelial Cells
Application
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Disease Modeling, Epithelial Cell Biology, Respiratory Research
Clone
Polyclonal
Gene ID
7082
Isotype
IgG

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human Tight Junction Protein 1 (ZO-1) Antibody, Polyclonal
Catalog #
100-0750, 100-0751, 100-0752
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human Tight Junction Protein 1 (ZO-1) Antibody, Polyclonal
Catalog #
100-0750, 100-0751, 100-0752
Lot #
All
Language
English

Educational Materials (6)

Brochure
Reliable Antibodies for Your Research
Brochure
PneumaCult™ Culture Media for Human Airway Epithelial Cells
Protocol
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at the Air-Liquid Interface
Wallchart
SnapShot: COVID-19
Webinar
Studying Respiratory Viruses with ALI Cultures
6:56
Studying Respiratory Viruses with ALI Cultures
Mini Review
Airway Epithelial Cells of the Human Respiratory System

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Confocal image of immunostained human bronchial epithelial cells.

Figure 1. Anti-Human Tight Junction Protein 1 (ZO-1) Antibody is Suitable for Staining Human Bronchial Epithelial Cells

Human bronchial epithelial cells (HBECs) cultured in PneumaCult™-ALI Medium were fixed, permeabilized, and labeled with Anti-Human Tight Junction Protein 1 (ZO-1) Antibody, Polyclonal, followed by goat anti-rabbit IgG (H+L), Alexa Fluor® 647. Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with rabbit IgG isotype control antibody, followed by goat anti-rabbit IgG (H+L), Alexa Fluor® 647 (with DAPI staining).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.