Anti-Human CD73 (Ecto-5'-Nucleotidase) Antibody, Clone AD2

Mouse monoclonal IgG1 antibody against human, rhesus, chimpanzee CD73

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Mouse monoclonal IgG1 antibody against human, rhesus, chimpanzee CD73
From: 145 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The AD2 antibody reacts with human CD73, a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein and ecto-5’-nucleotidase expressed on the surface of subsets of B and T cells, follicular dendritic cells, mesenchymal stem cells, endothelial cells, and epithelial cells. CD73 catalyzes the hydrolysis of 5’-adenosine monophosphate (AMP) to form the bioactive nucleoside adenosine, and plays a pivotal role in the activation of P1 adenosine receptors by regulating extracellular adenosine concentrations. CD73 also appears to function as a co-signaling molecule on T cells and as an adhesion molecule mediating lymphocyte interactions with the endothelium and follicular dendritic cells. CD73 is used as a marker for lymphocyte differentiation, its expression increasing during development. It is also a useful marker for identifying undifferentiated mesenchymal stem cells. CD73 is highly expressed in many types of human and mouse cancers, and has been implicated in the control of tumor growth.
Subtype:
Primary Antibodies
Target Antigen:
CD73
Alternative Names:
5'-nucleotidase, ecto (CD73), Ecto-5'-nucleotidase, L-VAP-2, NT5E
Reactive Species:
Human; Chimpanzee; Macaque; Rhesus
Conjugation:
APC; Biotin; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Mesenchymal Stem and Progenitor Cells
Application:
Flow Cytometry; Immunofluorescence; Immunohistochemistry
Area of Interest:
Stem Cell Biology
Clone:
AD2
Gene ID:
4907
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(1)
Brochure
Reliable Antibodies for Your Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Mammary Epithelial Cell Research
Tissue Dissociation and Isolation
Cell Culture and Assay
Characterization
Mesenchymal Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Culture and Cryopreservation
Differentiation
Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) and Anti-Human CD3 Antibody, Clone UCHT1, PE (Catalog #60011PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, APC and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (Catalog #60011AD). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, APC and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488.

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, Biotin, followed by streptavidin (SAV) APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC (Catalog #60011FI). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC.

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE (Catalog #60011PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody, FITC and Anti-Human CD3 Antibody, Clone UCHT1, PE.

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD73 Antibody, Clone AD2, PE and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (Catalog #60011AD). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with a mouse IgG1, kappa isotype control antibody and Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488.

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.