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Anti-Human CD45RO Antibody, Clone UCHL1

Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO

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Anti-Human CD45RO Antibody, Clone UCHL1

Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO

From: 153 USD
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Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO
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Overview

The UCHL1 antibody reacts with an extracellular epitope on CD45RO, which is the shortest isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 - 240 kDa. Excision of all three exons generates the ~180 kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The UCHL1 antibody has been used to identify T cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age. The UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.
Subtype
Primary Antibodies
Target Antigen
CD45RO
Alternative Names
B220, CD45, GP180, LCA, L-CA, LY5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Reactive Species
Chimpanzee, Common Marmoset, Human
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
Leukocytes, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human, Non-Human Primate
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microscopy, Western Blotting
Area of Interest
Immunology, Stem Cell Biology
Clone
UCHL1
Gene ID
5788
Isotype
IgG2a, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti-mouse IgG2a antibody, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti­mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on lymphocytes) and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC­173, followed by a rat anti-mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488 and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and an anti-human CD45RA antibody, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMSs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram) (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC and an anti-human CD45RA antibody, FITC.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, followed by streptavidin (SAV) APC and an anti-human CD45RA antibody, FITC (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC and an anti-human CD45RA antibody, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, FITC and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI) and an anti-human CD45RA antibody, APC.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE and an anti-human CD45RA antibody, APC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE and an anti-human CD45RA antibody, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PE
Catalog #
60097PE, 60097PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Biotin
Catalog #
60097BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, APC
Catalog #
60097AZ.1, 60097AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, FITC
Catalog #
60097FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488
Catalog #
60097AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1
Catalog #
60097
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5
Catalog #
60097PS.1, 60097PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PE
Catalog #
60097PE, 60097PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Biotin
Catalog #
60097BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, APC
Catalog #
60097AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, FITC
Catalog #
60097FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, APC
Catalog #
60097AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488
Catalog #
60097AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1
Catalog #
60097
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5
Catalog #
60097PS.1, 60097PS
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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