Anti-Human CD43 Antibody, Clone CD43-10G7

Mouse monoclonal IgG1 antibody against human CD43

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From: 113 USD

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Mouse monoclonal IgG1 antibody against human CD43
From: 113 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The CD43-10G7 antibody reacts with human CD43, a type I transmembrane sialoglycoprotein expressed on the surface of most human hematopoietic cells, including thymocytes, peripheral T cells, NK cells, monocytes, granulocytes, some B cells (activated B cells and an early B cell subset in bone marrow), plasma cells, and hematopoietic progenitor cells. Low expression levels are observed on platelets. CD43 exhibits different glycoforms in the range of ~95 - 135 kDa, depending on the cell type. It binds ligands such as CD54 and CD169 (Siglec-1) and plays regulatory roles in cell adhesion and activation, apoptosis and tumor progression, and is used a marker for discriminating normal from neoplastic T cells and B cells within tissues. Binding of the CD43-10G7 antibody to CD43 on neutrophils reportedly induces homotypic cell adhesion.
Subtype:
Primary Antibodies
Target Antigen:
CD43
Alternative Names:
Leukocyte sialoglycoprotein, Leukosialin, Ly-48, Sialophorin, W3/13
Reactive Species:
Human
Conjugation:
APC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
B Cells
Application:
Functional Assay; Flow Cytometry; Immunoprecipitation
Area of Interest:
Immunology
Clone:
CD43-10G7
Gene ID:
6693
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
Complete Antibody Listing
Brochure
HLA Antibodies for Purity Assessment
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC.

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.