Anti-Human CD38 Antibody, Clone HIT2

Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38

Anti-Human CD38 Antibody, Clone HIT2

Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38

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Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38
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Overview

The HIT2 antibody reacts with CD38, an ~45 kDa type II transmembrane glycoprotein expressed by many cell types, especially leukocytes. CD38 is an ectoenzyme (ADP-ribosyl hydrolase) with both cyclase and hydrolase activities, whereby it mediates lymphocyte activation, adhesion and metabolism. CD31 is a known ligand for the receptor. In humans, CD38 is expressed by the majority of hematopoietic cells at levels which vary according to the differentiation and activation status of the cells. It is highly expressed on activated T and B cells and plasma cells, and is also found at high levels in several tissues, including brain, kidney, liver, ovary, pancreas, placenta, testis, and some types of cancerous tissue.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human CD138 Positive Selection Kit II (Catalog #1877) and EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887).
Subtype
Primary Antibodies
Target Antigen
CD38
Alternative Names
ADP-ribosyl cyclase, T10
Reactive Species
Chimpanzee, Horse, Human
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Plasma
Species
Human, Non-Human Primate, Other
Application
Cell Isolation, CyTOF, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting
Area of Interest
Immunology, Stem Cell Biology
Clone
HIT2
Gene ID
952
Isotype
IgG1, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD38 Antibody, Clone HIT2, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60014BT
Lot #
All
Language
English
Catalog #
60014AD.1, 60014AD
Lot #
All
Language
English
Catalog #
60014
Lot #
All
Language
English
Catalog #
60014PE.1, 60014PE
Lot #
All
Language
English
Catalog #
60014FI, 60014FI.1
Lot #
All
Language
English
Catalog #
60014AZ.1, 60014AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014AD.1, 60014AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014PE.1, 60014PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014FI, 60014FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60014AZ.1, 60014AZ
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.