Anti-Human CD22 Antibody, Clone HIB22

Mouse monoclonal IgG1 antibody against human, chimpanzee CD22

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Mouse monoclonal IgG1 antibody against human, chimpanzee CD22
From: 138 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The HIB22 antibody reacts with CD22 (Siglec-2), a type I transmembrane glycoprotein expressed in the cytoplasm of pro-B and pre-B cells and on the surface of mature and activated B cells and B cell lymphomas, but not on plasma cells. CD22 functions as an inhibitory receptor for B cell receptor signaling and plays roles in modulating the activation threshold of B cells and controlling CD40 signaling. CD22 comprises two isoforms generated by alternative splicing: CD22α (~130 kDa) and CD22β (~140 kDa). Monomeric CD22β is the most abundant isoform but an αβ heterodimer is also expressed. CD22 is a member of the sialoadhesion subgroup within the immunoglobulin superfamily, with an N-terminal V-type Ig domain followed by six C2-type Ig domains. Binding of the HIB22 antibody reportedly requires the presence of the two N-terminal-most domains. Both the cytoplasmic and surface-bound forms of CD22 are recognized by the HIB22 antibody and its binding does not inhibit CD22-mediated cyto-adhesion.
Subtype:
Primary Antibodies
Target Antigen:
CD22
Alternative Names:
B-lymphocyte cell adhesion molecule, BLCAM, Leu-14, Lyb8, Sialic acid-binding Ig-like lectin 2, Siglec-2
Reactive Species:
Chimpanzee; Human
Conjugation:
APC; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
B Cells
Application:
ELISA; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation
Area of Interest:
Immunology
Clone:
HIB22
Gene ID:
933
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
Complete Antibody Listing
Brochure
HLA Antibodies for Purity Assessment
Brochure
Tools For Your Immunology Research
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 4. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.