Annexin V

A cellular protein for detection of apoptotic cells

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A cellular protein for detection of apoptotic cells
From: 89 USD

Overview

Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium. This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment. Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment.
Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002). The process of PS translocation occurs prior to the loss of membrane integrity. Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye.
Conjugation:
APC; FITC; PE
Application:
Flow Cytometry
Area of Interest:
Immunology

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC Annexin V and 7-AAD.

Figure 1. Data for Annexin V, APC

Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).

Figure showing flow cytometry analysis of Con-A stimulated (3 days) C57BL/6 mouse splenocytes labeled with Anti-Mouse CD152 (CTLA-4) Antibody, Clone UC10-4F10-11, PE or an Armenian hamster IgG, PE isotype control antibody.

Figure 2. Data for Annexin V, FITC

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC Annexin V and 7-AAD.

Figure showing flow cytometry analysis of Con A-stimulated (3 days) C57BL/6 mouse splenocytes labeled with Anti-Mouse CD152 (CTLA-4) Antibody, Clone UC10-4F10-11, followed by a goat anti-Armenian hamster IgG antibody, FITC or an Armenian hamster IgG isotype control antibody, followed by a goat anti-Armenian hamster IgG, FITC.

Figure 3. Data for Annexin V, PE

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE Annexin V and 7-AAD.

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