EasySep™ Cell Isolation & Cell Separation Technology
EasySep™ Magnetic Cell Separation
The Easy Choice for Simple Cell Isolation
Scientific research can be demanding, so working efficiently is essential. You need the right tools that not only save time but also make your workflow easier. That’s why our scientists created EasySep™, a simpler, easier way to isolate the cells you need. This versatile technology combines the specificity of monoclonal antibodies with a column-free magnetic system, allowing you to get highly purified cells in as little as 8 minutes. Whether you're new to cell separation or an experienced researcher, choose EasySep™ to simplify your workflow—so you can focus on achieving great science.
Curious about how EasySep™ can simplify your research? Experience how easy it is by requesting a sample. Say goodbye to complicated protocols and time-consuming, column-based cell isolations. Make the easy choice and move your research forward faster.
Why Use EasySep™ to Isolate Cells?
- Isolate cells in as little as 8 minutes with a simple pour.
- Achieve up to 99% cell purities with high recoveries.
- Obtain viable, functional cells without the need for columns and washes.
- Isolate cells from virtually any sample source, including whole blood and leukopaks.
- Widely used in published research for over 20 years, supporting diverse downstream applications.
How Does EasySep™ Magnetic Cell Separation Work?
Cells are targeted for either removal (negative selection and depletion) or positive selection using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ cell separation magnet, while untouched cells can be simply poured or pipetted off into a new tube. EasySep™ methods are column-free, reducing mechanical stress on cells and preserving their functionality and viability.
Figure 1. Comparison of Two Different Immunomagnetic Cell Isolation Protocols
Steps involved in the isolation of human T cells by negative selection using two different immunomagnetic cell isolation technologies are shown: (A) column-free EasySep™ technology (time taken may vary depending on the type of magnet used) and (B) a commercially available column-based technology (*elution time varies).
Immunomagnetic-based EasySep™ technology supports cell isolation from a wide range of species (e.g. human, mouse, non-human primate, and rat) and sample sources (e.g. peripheral blood mononuclear cells (PBMCs), leukopaks, whole blood, splenocytes, lymph nodes, cord blood, and bone marrow).* Learn more about how EasySep™ magnetic cell separation technology works and key factors to consider when choosing a negative or positive selection strategy for your research needs.
EasySep™–A Trusted Cell Isolation Method for Over 20 Years
EasySep™ has been extensively referenced in more than 6,500 peer-reviewed studies for over 20 years, demonstrating consistent performance across various cell types and downstream applications, including flow cytometry, cell culture, cell expansion, DNA/RNA extraction, sequencing, cell-based assays, and more. The effectiveness and versatility of EasySep™ technology has led to widespread adoption in fields of study such as immunology, cancer, stem cell biology, and cell therapy research. Browse our Immunology Features highlighting major scientific advances supported by EasySep™ products.
Thousands of labs worldwide already trust EasySep™ for its speed and simplicity in isolating highly purified cells. Transitioning to EasySep™ for your cell isolation needs is easier than you think. Get started now by requesting a sample or hear more from EasySep™ users about their research.
EasySep™ Frequently Asked Questions (FAQs) and Product Selection Resources
Find quick answers to frequently asked questions (FAQs) about EasySep™ cell isolation, product compatibility, sample requests, and more. Alternatively, explore the information below to help you find the right EasySep™ product for your application.
Looking for additional EasySep™ technology and protocol guidance to get the best results in your experiments? Explore our comprehensive range of EasySep™ technical tips, or browse our Methods Library for more resources and cell separation protocols by negative selection and positive selection. Alternatively, please contact us at techsupport@stemcell.com for assistance.
Explore EasySep™ Products for Human, Mouse, and Other Species
Explore a wide range of EasySep™ products organized by species and application, making it easier than ever to find the right isolation kit for your research. Don’t see a product for your application? Custom cell separation kits can be tailored to isolate specific or rare cell types not addressed by standard products. For more information or to discuss your specific cell isolation requirements, please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com.
EasySep™ Human Cell Separation Kits
EasySep™ Human Cell Isolation Kits
Protocol Time:
Selection Method:
Features/Advantages:
- Easy to use
- High purity (up to 98%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
EasySep™ Human Positive Selection Kits
Protocol Time:
Selection Method:
Features/Advantages:
- Easy to use
- High purity (up to 99%)
- Column-free
- Can be fully automated using
EasySep™ Direct Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Easy to use
- 99.9% RBC depletion without density gradient centrifugation, lysis or sedimentation
- High purity (up to 99%)
- Column-free
- Untouched cells immediately ready for downstream analysis
EasySep™ Release Cell Separation Kits
Protocol Time:
Selection Method:
Features/Advantages:
- Easy to use
- High purity (up to 99%)
- Column-free
- Magnetic particle-free cells
- Flexibility to perform sequential positive selections to isolate unique and rare cell types
*Certain products are only available in select territories. Please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.
EasySep™ Mouse and Rat Cell Separation Kits
EasySep™ Mouse Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Easy and fast
- High purity (up to 98%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
EasySep™ Mouse Positive Selection Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Easy and fast
- High purity (up to 98%)
- Column-free
- Can be fully automated using
EasySep™ Rat Cell Isolation Kits
Typical Sample Source:
Protocol Time:
Selection Method:
Features/Advantages:
- Easy and fast
- High purity (up to 98%)
- Column-free
- Untouched cells immediately ready for downstream analysis
- Can be fully automated using
How to Choose the Right EasySep™ Magnet
EasySep™ magnets are specifically designed for cell separation using EasySep™ reagents, eliminating the need for columns. They generate a high-gradient magnetic field within the interior cavity, effectively separating cells labeled with EasySep™ Magnetic Particles. EasySep™ magnets are available in a range of different sizes, allowing researchers to process a variety of sample volumes and cell numbers to suit their downstream application needs. Use this comparison table to help you choose the right magnet for your needs.
Automated Cell Separation with RoboSep™
EasySep™ immunomagnetic isolation can also be automated using RoboSep™ instruments, offering true walk-away automation options for labs requiring higher throughput, providing the same ease and reliability on a larger scale. Minimize sample handling and perform simultaneous and sequential cell isolations from multiple samples using RoboSep™-S and RoboSep™-16 instruments.
On-Demand Human Immune Cell Isolation Course
Learn how to effectively process human samples and isolate highly pure target cells with EasySep™ in this free self-paced course.
Get Started with EasySep™ Today
Are you ready to make cell separation easy? Whether you’re ready to buy, want to request a free sample, or need a demo, we’re here to help simplify your research journey. Choose Your Next Steps:
Request a Sample
Discover the ease and reliability of EasySep™ products.
Schedule a Demo
Book a personalized demonstration to see the Easy 250 EasySep™ magnet in action.
Contact Product & Scientific Support
Get protocols, best practices, and product applications.
Technical Resources
Access technical bulletins, webinars, and more.
Ways to Order
We make ordering easy.
On-Demand Courses
Watch step-by-step lab and lecture videos to guide you through full protocols.
Start achieving great science, the easy way.
Related Resources
Magnetic Cell Separation
Find answers to three commonly asked questions about immunomagnetic cell separation.
Choosing a Positive or Negative Selection Approach
Read more about antibody labeling-based cell separation methods, including immunomagnetic and immunodensity cell separation.
Processing a Leukopak for Downstream Cell Isolation
Learn how to process a fresh or frozen leukopak sample by following this step-by-step protocol video.
Manual Large-Volume Cell Isolation
Isolate human immune cells from samples of up to 225 mL in a single cell isolation step with the Easy 250 EasySep™ Magnet.
Fully Automated Immunomagnetic Cell Isolation
RoboSep™ instruments fully automate all cell labeling and separation steps of the EasySep™ procedure, minimizing sample handling and freeing up time.
Find the Right Antibody for Your Applications
Browse our complete antibody product listing page to select high-quality primary and secondary antibodies verified to work with EasySep™ products in specific applications.
*Certain products are only available in select territories. Please contact your local sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.
