Pluripotent Stem Cell Training

Hands-on training to support human pluripotent stem cell research

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From: 1,499 USD

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Hands-on training to support human pluripotent stem cell research
From: 1,499 USD

Course Overview

Learn the techniques and protocols to successfully derive and maintain high-quality human induced pluripotent stem (hiPS) cells from somatic cells and their differentiation to specialized cell types.

Customize your training by choosing from the course topics available:

  • Reprogramming somatic cells to hiPS cells
  • Maintaining hiPS cells and human embryonic stem (hES) cells
  • *Differentiating hES and hiPS cells towards one of the following:
    • Cardiomyocytes (Option A)
    • Hematopoietic progenitor cells (Option B)
    • Neural progenitor cells (Option C)

*Select your differentiation topic of choice (Option A, B, or C) in the Sign Up form.
Please see Agenda for more information.

Participants Will Receive

  • Course booklet and USB key with instructional materials
  • Tote bag with sample-sized products (where applicable)
  • Certificate following successful completion of the course
  • Lunch and refreshments on each day and dinner hosted on one of the evenings
Training and Education
Training Programs
Stem Cell Biology; Drug Discovery and Toxicity Testing
Embryonic Stem Cells and iPS Cells
Human
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
The Netherlands:
Leiden University Medical Center
Building 2
Einthovenweg 20, 2333 ZC Leiden
Leiden, NL

Speakers

Speakers will vary depending on course topic, date, and location.

Arwen Hunter, PhD
Senior Scientist (Pluripotent Group), Research and Development
STEMCELL Technologies
Leon Chew, MSc
Scientist (Neural Group), Research and Development
STEMCELL Technologies
Melanie Kardel, PhD
Senior Scientist (Pluripotent Group), Research and Development
STEMCELL Technologies
Vincenzo Macri, PhD
Senior Scientist (Myogenic Group), Research and Development
STEMCELL Technologies
Wing Chang, PhD
Director, Research and Development UK
STEMCELL Technologies
Christine Mummery, PhD
Chair of the Department of Anatomy and Embryology
Leiden University Medical Center
(Guest speaker at Leiden, The Netherlands course)

Course Agenda (Tentative)

Note that each day will end at approximately 4:30 pm with the course dinner taking place on the evening of the Maintaining Human Pluripotent Stem Cells (hPSCs) day.
All course options followed by wrap-up which includes a quiz, evaluation, and certificate presentation.


Reprogramming of Somatic Cells to Induced Pluripotent Stem (iPS) Cells

  • Lecture: Isolation and Reprogramming of Somatic Cells to iPS Cells
  • Practical Session: Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
    • Overview of EasySep™ cell separation system
    • Demonstrate preparation of PBMCs using SepMate™
    • Observe culture-expanded blood cells in StemSpan™ SFEM
  • Practical Session: Reprogramming of Somatic Cells to iPS Cells
    • Demonstrate transfection of human fibroblasts using ReproRNA™-OKSGM
    • Observe reprogrammed cells cultured in ReproTeSR™
  • Practical Session: Selection and Picking of iPS Cell Colonies

Maintaining Human Pluripotent Stem Cells (hPSCs)

  • Lecture: Feeder-Free Culture Systems for hPSCs
  • Practical Session: Evaluation of the Quality of hPSC Cultures and Plate Coating
    • Observe time course of hPSC cultures and evaluate colony morphology
    • Practice plate coating with Matrigel® or
      Vitronectin XF™
  • Practical Session: Passaging and Long-Term Maintenance of hPSCs
    • Practice enzyme-free passaging of hPSCs using
      Gentle Cell Dissociation Reagent or ReLeSR™
  • Practical Session: Cryopreservation and Thawing of hPSCs
  • Course Dinner (Courtesy of STEMCELL Technologies)

Differentiating hPSCs

(Select Option in Sign-Up Form)

Option A: Differentiation towards Cardiomyocytes
  • Lecture: Differentiation of hPSCs towards Cardiomyocytes
  • Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
  • Practical Session: Preparation of hPSCs as Single Cells for Cardiomyocyte Differentiation
  • Practical Session: Observation of hPSC-Derived Cardiomyocytes
    • Observe time course of hPSC-derived cardiomyocytes
    • Dissociation and replating of hPSC-derived cardiomyocytes for downstream applications
  • Practical Session: Cryopreservation and Thawing of hPSC-Derived Cardiomyocytes and Downstream Applications
Option B: Differentiation towards Hematopoietic Progenitor Cells (HPCs)
  • Lecture: Differentiation of hPSCs towards Hematopoietic Progenitor Cells
  • Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
  • Practical Session: Observation of hPSC-Derived HPCs
  • Practical Session: HPCs for Downstream Applications
    • Harvest HPCs from culture supernatant
    • Analyze hPSC-derived HPCs using the colony-forming unit (CFU) assay
  • Practical Session: Identification and Counting of Colonies in the CFU Assay
Option C: Differentiation towards Neural Progenitor Cells (NPCs)
  • Lecture: Differentiation of hPSCs towards Neural Progenitor Cells and Neuronal Cell Types
  • Practical Session: Generation of Embryoid Bodies (EBs) Using AggreWell™ and Preparation of Single Cell hPSCs for Monolayer-Based Protocols
  • Practical Session: Differentiation of hPSCs to NPCs
    • Examine neural aggregates in AggreWell™ and harvest EBs
    • Observe time course of differentiated NPCs
    • Assess neural induction by scoring neural rosette formation from EBs
  • Lecture: Introduction to the Establishment and Maturation of hPSC-Derived Cerebral Organoids

Accommodations

Vancouver, CA

Pan Pacific Vancouver
300 - 999 Canada Place, Vancouver, BC V6C 3B5
Sheraton Wall Centre
1088 Burrard St, Vancouver, BC V6Z 2R9
Hampton Inn & Suites by Hilton
111 Robson St, Vancouver, BC V6B 2A8
Executive Hotel Le Soleil
567 Hornby St, Vancouver, BC V6C 2E8

Cambridge, UK

Holiday Inn Cambridge
Lakeview Bridge Road, Impington, Cambridge, CB24 9PH

Leiden, NL

Holiday Inn Leiden
Haagse Schouwweg 10, 2332 KG, Leiden, Netherlands
IBIS Leiden Centre
Stationsplein 240-242, 2312 AR Leiden, Netherlands
City Resort Hotel Leiden
Bargelaan 180, 2333 CW Leiden, Netherlands

Please contact education@stemcell.com for more information on seasonal rates (when applicable).

Policy

Travel Visas

Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.

Travel Visa Requirements

Canada
United Kingdom
Netherlands

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

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